Characterisation of Bone Beneficial Components from Australian Wallaby Bone.

Lao, W; Jin, X; Tan, Y; Xiao, L; Padula, MP; Bishop, DP; Reedy, B; Ong, M; Kamal, MA; Qu, X

Characterisation of Bone Beneficial Components from Australian Wallaby Bone.

Lao, W

Jin, X Tan, Y

Xiao, L Padula, MP

Bishop, DP

Reedy, B

Ong, M Kamal, MA

Qu, X

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Publication Type:: Journal Article
Citation:: Medicines (Basel), 2016, 3 (3)
Issue Date:: 2016-08-26

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Field	Value	Language
dc.contributor.author	Lao, W https://orcid.org/0000-0003-4125-3592	en_US
dc.contributor.author	Jin, X	en_US
dc.contributor.author	Tan, Y https://orcid.org/0000-0001-9221-330X	en_US
dc.contributor.author	Xiao, L	en_US
dc.contributor.author	Padula, MP https://orcid.org/0000-0002-8283-0643	en_US
dc.contributor.author	Bishop, DP https://orcid.org/0000-0002-6533-4410	en_US
dc.contributor.author	Reedy, B https://orcid.org/0000-0003-1797-8091	en_US
dc.contributor.author	Ong, M	en_US
dc.contributor.author	Kamal, MA https://orcid.org/0000-0003-0088-0565	en_US
dc.contributor.author	Qu, X	en_US
dc.date.available	2016-08-23	en_US
dc.date.issued	2016-08-26	en_US
dc.identifier.citation	Medicines (Basel), 2016, 3 (3)	en_US
dc.identifier.issn	2305-6320	en_US
dc.identifier.uri	http://hdl.handle.net/10453/72044
dc.description.abstract	Background: Osteoporosis is a condition in which the bones become brittle, increasing the risk of fractures. Complementary medicines have traditionally used animal bones for managing bone disorders, such as osteoporosis. This study aimed to discover new natural products for these types of conditions by determining mineral and protein content of bone extracts derived from the Australian wallaby. Methods: Inductively coupled plasma-mass spectrometry and Fourier transform infrared spectroscopic analysis were used for mineral tests, proteome analysis was using LC/MS/MS and the effects of wallaby bone extracts (WBE)s on calcium deposition and alkaline phosphatase activity were evaluated in osteogenic cells derived from adipose tissue-derived stem cells (ADSCs). Results: Concentrations of calcium and phosphorus were 26.21% and 14.72% in WBE respectively. Additionally, minerals found were wide in variety and high in concentration, while heavy metal concentrations of aluminium, iron, zinc and other elements were at safe levels for human consumption. Proteome analysis showed that extracts contained high amounts of bone remodelling proteins, such as osteomodulin, osteopontin and osteoglycin. Furthermore, in vitro evaluation of WBEs showed increased deposition of calcium in osteoblasts with enhanced alkaline phosphatase activity in differentiated adipose-derived stem cells. Conclusion: Our results demonstrate that wallaby bone extracts possess proteins and minerals beneficial for bone metabolism. WBEs may therefore be used for developing natural products for conditions such as osteoporosis and further investigation to understand biomolecular mechanism by which WBEs prevent osteoporosis is warranted.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Medicines (Basel)	en_US
dc.relation.isbasedon	10.3390/medicines3030023	en_US
dc.title	Characterisation of Bone Beneficial Components from Australian Wallaby Bone.	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.location.activity	Switzerland	en_US
utslib.for	060114 Systems Biology	en_US
utslib.for	110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)	en_US
utslib.for	060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	3	en_US
pubs.publication-status	Published online	en_US
pubs.volume	3	en_US

Abstract:

Background: Osteoporosis is a condition in which the bones become brittle, increasing the risk of fractures. Complementary medicines have traditionally used animal bones for managing bone disorders, such as osteoporosis. This study aimed to discover new natural products for these types of conditions by determining mineral and protein content of bone extracts derived from the Australian wallaby. Methods: Inductively coupled plasma-mass spectrometry and Fourier transform infrared spectroscopic analysis were used for mineral tests, proteome analysis was using LC/MS/MS and the effects of wallaby bone extracts (WBE)s on calcium deposition and alkaline phosphatase activity were evaluated in osteogenic cells derived from adipose tissue-derived stem cells (ADSCs). Results: Concentrations of calcium and phosphorus were 26.21% and 14.72% in WBE respectively. Additionally, minerals found were wide in variety and high in concentration, while heavy metal concentrations of aluminium, iron, zinc and other elements were at safe levels for human consumption. Proteome analysis showed that extracts contained high amounts of bone remodelling proteins, such as osteomodulin, osteopontin and osteoglycin. Furthermore, in vitro evaluation of WBEs showed increased deposition of calcium in osteoblasts with enhanced alkaline phosphatase activity in differentiated adipose-derived stem cells. Conclusion: Our results demonstrate that wallaby bone extracts possess proteins and minerals beneficial for bone metabolism. WBEs may therefore be used for developing natural products for conditions such as osteoporosis and further investigation to understand biomolecular mechanism by which WBEs prevent osteoporosis is warranted.

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http://hdl.handle.net/10453/72044