Mechanisms involved in acquisition of bla<inf>NDM</inf> genes by IncA/C<inf>2</inf> and IncFII<inf>Y</inf> plasmids

Wailan, AM; Sidjabat, HE; Yam, WK; Alikhan, NF; Petty, NK; Sartor, AL; Williamson, DA; Forde, BM; Schembri, MA; Beatson, SA; Paterson, DL; Walsh, TR; Partridge, SR

Mechanisms involved in acquisition of bla<inf>NDM</inf> genes by IncA/C<inf>2</inf> and IncFII<inf>Y</inf> plasmids

Wailan, AM Sidjabat, HE Yam, WK Alikhan, NF Petty, NK

Sartor, AL Williamson, DA Forde, BM Schembri, MA Beatson, SA

Paterson, DL Walsh, TR Partridge, SR

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Publication Type:: Journal Article
Citation:: Antimicrobial Agents and Chemotherapy, 2016, 60 (7), pp. 4082 - 4088
Issue Date:: 2016-07-01

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Field	Value	Language
dc.contributor.author	Wailan, AM	en_US
dc.contributor.author	Sidjabat, HE	en_US
dc.contributor.author	Yam, WK	en_US
dc.contributor.author	Alikhan, NF	en_US
dc.contributor.author	Petty, NK https://orcid.org/0000-0001-6528-9886	en_US
dc.contributor.author	Sartor, AL	en_US
dc.contributor.author	Williamson, DA	en_US
dc.contributor.author	Forde, BM	en_US
dc.contributor.author	Schembri, MA	en_US
dc.contributor.author	Beatson, SA https://orcid.org/0000-0002-1806-3283	en_US
dc.contributor.author	Paterson, DL	en_US
dc.contributor.author	Walsh, TR	en_US
dc.contributor.author	Partridge, SR	en_US
dc.date.available	2016-04-22	en_US
dc.date.issued	2016-07-01	en_US
dc.identifier.citation	Antimicrobial Agents and Chemotherapy, 2016, 60 (7), pp. 4082 - 4088	en_US
dc.identifier.issn	0066-4804	en_US
dc.identifier.uri	http://hdl.handle.net/10453/88351
dc.description.abstract	Copyright © 2016, American Society for Microbiology. All Rights Reserved. blaNDM genes confer carbapenem resistance and have been identified on transferable plasmids belonging to different incompatibility (Inc) groups. Here we present the complete sequences of four plasmids carrying a blaNDM gene, pKP1-NDM-1, pEC2-NDM-3, pECL3-NDM-1, and pEC4-NDM-6, from four clinical samples originating from four different patients. Different plasmids carry segments that align to different parts of the blaNDM region found on Acinetobacter plasmids. pKP1-NDM-1 and pEC2-NDM-3, from Klebsiella pneumoniae and Escherichia coli, respectively, were identified as type 1 IncA/C2 plasmids with almost identical backbones. Different regions carrying blaNDM are inserted in different locations in the antibiotic resistance island known as ARI-A, and ISCR1 may have been involved in the acquisition of blaNDM-3 by pEC2-NDM-3. pECL3-NDM-1 and pEC4-NDM-6, from Enterobacter cloacae and E. coli, respectively, have similar IncFIIY backbones, but different regions carrying blaNDM are found in different locations. Tn3-derived inverted-repeat transposable elements (TIME) appear to have been involved in the acquisition of blaNDM-6 by pEC4-NDM-6 and the rmtC 16S rRNA methylase gene by IncFIIY plasmids. Characterization of these plasmids further demonstrates that even very closely related plasmids may have acquired blaNDM genes by different mechanisms. These findings also illustrate the complex relationships between antimicrobial resistance genes, transposable elements, and plasmids and provide insights into the possible routes for transmission of blaNDM genes among species of the Enterobacteriaceae family.	en_US
dc.relation.ispartof	Antimicrobial Agents and Chemotherapy	en_US
dc.relation.isbasedon	10.1128/AAC.00368-16	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Acinetobacter	en_US
dc.subject.mesh	Enterobacter cloacae	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Klebsiella pneumoniae	en_US
dc.subject.mesh	Carbapenems	en_US
dc.subject.mesh	beta-Lactamases	en_US
dc.subject.mesh	DNA Transposable Elements	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Microbial Sensitivity Tests	en_US
dc.subject.mesh	Drug Resistance, Multiple, Bacterial	en_US
dc.subject.mesh	Plasmids	en_US
dc.title	Mechanisms involved in acquisition of bla<inf>NDM</inf> genes by IncA/C<inf>2</inf> and IncFII<inf>Y</inf> plasmids	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	60	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	60	en_US

Abstract:

Copyright © 2016, American Society for Microbiology. All Rights Reserved. blaNDM genes confer carbapenem resistance and have been identified on transferable plasmids belonging to different incompatibility (Inc) groups. Here we present the complete sequences of four plasmids carrying a blaNDM gene, pKP1-NDM-1, pEC2-NDM-3, pECL3-NDM-1, and pEC4-NDM-6, from four clinical samples originating from four different patients. Different plasmids carry segments that align to different parts of the blaNDM region found on Acinetobacter plasmids. pKP1-NDM-1 and pEC2-NDM-3, from Klebsiella pneumoniae and Escherichia coli, respectively, were identified as type 1 IncA/C2 plasmids with almost identical backbones. Different regions carrying blaNDM are inserted in different locations in the antibiotic resistance island known as ARI-A, and ISCR1 may have been involved in the acquisition of blaNDM-3 by pEC2-NDM-3. pECL3-NDM-1 and pEC4-NDM-6, from Enterobacter cloacae and E. coli, respectively, have similar IncFIIY backbones, but different regions carrying blaNDM are found in different locations. Tn3-derived inverted-repeat transposable elements (TIME) appear to have been involved in the acquisition of blaNDM-6 by pEC4-NDM-6 and the rmtC 16S rRNA methylase gene by IncFIIY plasmids. Characterization of these plasmids further demonstrates that even very closely related plasmids may have acquired blaNDM genes by different mechanisms. These findings also illustrate the complex relationships between antimicrobial resistance genes, transposable elements, and plasmids and provide insights into the possible routes for transmission of blaNDM genes among species of the Enterobacteriaceae family.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/74866