Analysis of sedimentation equilibrium results obtained with indefinitely self-associating systems using a procedure based on laplace transformation
- Publication Type:
- Journal Article
- Journal of Physical Chemistry, 1976, 80 (10), pp. 1071 - 1075
- Issue Date:
Equations are developed in closed form which permit the simulation of the distribution of total concentration vs. radial distance obtainable in the sedimentation equilibrium of a system of specified initial concentration undergoing indefinite self-association. Simulated distributions obtained with a variety of systems involving one or more equilibrium constants are used to test an analysis procedure which fits the distribution to a function capable of inverse Laplace transformation and leads to a specification of the relative amounts of the species in the cell as a function of their molecular weights. It is shown that such results may be related to the equilibrium concentrations of oligomeric forms at each radial distance, thereby permitting successive equilibrium constants appropriate to the indefinite self-association to be estimated.
Please use this identifier to cite or link to this item: