Solid-phase extraction in combination with GC/MS for the quantification of free fatty acids in adipocere

Notter, SJ; Stuart, BH; Dent, BB; Keegan, J

Solid-phase extraction in combination with GC/MS for the quantification of free fatty acids in adipocere

Notter, SJ Stuart, BH

Dent, BB Keegan, J

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Publication Type:: Journal Article
Citation:: European Journal of Lipid Science and Technology, 2008, 110 (1), pp. 73 - 80
Issue Date:: 2008-02-06

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Field	Value	Language
dc.contributor.author	Notter, SJ	en_US
dc.contributor.author	Stuart, BH https://orcid.org/0000-0001-9540-4435	en_US
dc.contributor.author	Dent, BB	en_US
dc.contributor.author	Keegan, J	en_US
dc.date.issued	2008-02-06	en_US
dc.identifier.citation	European Journal of Lipid Science and Technology, 2008, 110 (1), pp. 73 - 80	en_US
dc.identifier.issn	1438-7697	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8493
dc.description.abstract	Current research investigating the effect of specific aquatic microenvironments on the formation of adipocere using domesticated pigs (Sus scrofa) has demonstrated the need for a fast and reliable method to separate and identify fatty acids present in adipocere. Adipocere is defined as a late-stage post-mortem decomposition product consisting of a mixture of free fatty acids (FPA), which have formed under favorable conditions due to the hydrolysis of triglycerides in adipose tissue. Whilst good separations of adipocere lipids have been achieved using TLC, this method is time consuming when processing large numbers of samples. This paper describes a rapid and simple method for the extraction, identification and quantification of FFA commonly found in adipocere, by solid-phase extraction (SPE) using aminopropyl disposable columns in combination with GC/MS. The recoveries of FFA associated with adipocere were all above 90%, with coefficients of variation below 10%, indicating that the technique was reproducible. The limits of quantification were registered at levels of parts per million. Standard curves were linear over the range of 50-1000 μg/mL, with all correlation coefficient values greater than 0.998. A marked increase in concentration of saturated fatty acids was observed during adipocere formation, ranging from 20 to 55% for palmitic acid, 13 to 23% for stearic acid and 2.8 to 4.1% for myristic acid. These results demonstrate the suitability of aminopropyl disposable SPE columns to efficiently and rapidly isolate FFA from adipocere prior to quantitative GC/MS analysis. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.	en_US
dc.relation.ispartof	European Journal of Lipid Science and Technology	en_US
dc.relation.isbasedon	10.1002/ejlt.200700159	en_US
dc.subject.classification	Nutrition & Dietetics	en_US
dc.title	Solid-phase extraction in combination with GC/MS for the quantification of free fatty acids in adipocere	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	110	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	110	en_US

Abstract:

Current research investigating the effect of specific aquatic microenvironments on the formation of adipocere using domesticated pigs (Sus scrofa) has demonstrated the need for a fast and reliable method to separate and identify fatty acids present in adipocere. Adipocere is defined as a late-stage post-mortem decomposition product consisting of a mixture of free fatty acids (FPA), which have formed under favorable conditions due to the hydrolysis of triglycerides in adipose tissue. Whilst good separations of adipocere lipids have been achieved using TLC, this method is time consuming when processing large numbers of samples. This paper describes a rapid and simple method for the extraction, identification and quantification of FFA commonly found in adipocere, by solid-phase extraction (SPE) using aminopropyl disposable columns in combination with GC/MS. The recoveries of FFA associated with adipocere were all above 90%, with coefficients of variation below 10%, indicating that the technique was reproducible. The limits of quantification were registered at levels of parts per million. Standard curves were linear over the range of 50-1000 μg/mL, with all correlation coefficient values greater than 0.998. A marked increase in concentration of saturated fatty acids was observed during adipocere formation, ranging from 20 to 55% for palmitic acid, 13 to 23% for stearic acid and 2.8 to 4.1% for myristic acid. These results demonstrate the suitability of aminopropyl disposable SPE columns to efficiently and rapidly isolate FFA from adipocere prior to quantitative GC/MS analysis. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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http://hdl.handle.net/10453/8493