Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa

Whitchurch, CB; Leech, AJ; Young, MD; Kennedy, D; Sargent, JL; Bertrand, JJ; Semmler, ABT; Mellick, AS; Martin, PR; Alm, RA; Hobbs, M; Beatson, SA; Huang, B; Nguyen, L; Commolli, JC; Engel, JN; Darzins, A; Mattick, JS

Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa

Leech, AJ Young, MD Kennedy, D Sargent, JL Bertrand, JJ Semmler, ABT Mellick, AS Martin, PR Alm, RA Hobbs, M Beatson, SA Huang, B Nguyen, L Commolli, JC Engel, JN Darzins, A Mattick, JS

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Publication Type:: Journal Article
Citation:: Molecular Microbiology, 2004, 52 (3), pp. 873 - 893
Issue Date:: 2004-05-01

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Field	Value	Language
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Leech, AJ	en_US
dc.contributor.author	Young, MD	en_US
dc.contributor.author	Kennedy, D	en_US
dc.contributor.author	Sargent, JL	en_US
dc.contributor.author	Bertrand, JJ	en_US
dc.contributor.author	Semmler, ABT	en_US
dc.contributor.author	Mellick, AS	en_US
dc.contributor.author	Martin, PR	en_US
dc.contributor.author	Alm, RA	en_US
dc.contributor.author	Hobbs, M	en_US
dc.contributor.author	Beatson, SA	en_US
dc.contributor.author	Huang, B	en_US
dc.contributor.author	Nguyen, L	en_US
dc.contributor.author	Commolli, JC	en_US
dc.contributor.author	Engel, JN	en_US
dc.contributor.author	Darzins, A	en_US
dc.contributor.author	Mattick, JS	en_US
dc.date.issued	2004-05-01	en_US
dc.identifier.citation	Molecular Microbiology, 2004, 52 (3), pp. 873 - 893	en_US
dc.identifier.issn	0950-382X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8598
dc.description.abstract	Virulence of the opportunistic pathogen Pseudomonas aeruginosa involves the coordinate expression of a wide range of virulence factors including type IV pili which are required for colonization of host tissues and are associated with a form of surface translocation termed twitching motility. Twitching motility in P. aeruginosa is controlled by a complex signal transduction pathway which shares many modules in common with chemosensory systems controlling flagella rotation in bacteria and which is composed, in part, of the previously described proteins PilG, PilH, Pill, PilJ and PilK. Here we describe another three components of this pathway: ChpA, ChpB and ChpC, as well as two downstream genes, ChpD and ChpE, which may also be involved. The central component of the pathway, ChpA, possesses nine potential sites of phosphorylation: six histidine-containing phosphotransfer (HPt) domains, two novel serine- and threonine-containing phosphotransfer (SPt, TPt) domains and a CheY-like receiver domain at its C-terminus, and as such represents one of the most complex signalling proteins yet described in nature. We show that the Chp chemosensory system controls twitching motillty and type IV pill biogenesis through control of pili assembly and/or retraction as well as expression of the pilin subunit gene pilA. The Chp system is also required for full virulence in a mouse model of acute pneumonia.	en_US
dc.relation.ispartof	Molecular Microbiology	en_US
dc.relation.isbasedon	10.1111/j.1365-2958.2004.04026.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Fimbriae, Bacterial	en_US
dc.subject.mesh	Epithelial Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred BALB C	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Fimbriae Proteins	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Signal Transduction	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Movement	en_US
dc.subject.mesh	Multigene Family	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	52	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000220941400023	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	52	en_US

Abstract:

Virulence of the opportunistic pathogen Pseudomonas aeruginosa involves the coordinate expression of a wide range of virulence factors including type IV pili which are required for colonization of host tissues and are associated with a form of surface translocation termed twitching motility. Twitching motility in P. aeruginosa is controlled by a complex signal transduction pathway which shares many modules in common with chemosensory systems controlling flagella rotation in bacteria and which is composed, in part, of the previously described proteins PilG, PilH, Pill, PilJ and PilK. Here we describe another three components of this pathway: ChpA, ChpB and ChpC, as well as two downstream genes, ChpD and ChpE, which may also be involved. The central component of the pathway, ChpA, possesses nine potential sites of phosphorylation: six histidine-containing phosphotransfer (HPt) domains, two novel serine- and threonine-containing phosphotransfer (SPt, TPt) domains and a CheY-like receiver domain at its C-terminus, and as such represents one of the most complex signalling proteins yet described in nature. We show that the Chp chemosensory system controls twitching motillty and type IV pill biogenesis through control of pili assembly and/or retraction as well as expression of the pilin subunit gene pilA. The Chp system is also required for full virulence in a mouse model of acute pneumonia.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/8598