Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways

Whitchurch, CB; Beatson, SA; Comolli, JC; Jakobsen, T; Sargent, JL; Bertrand, JJ; West, J; Klausen, M; Waite, LL; Kang, PJ; Tolker-Nielsen, T; Mattick, JS; Engel, JN

Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways

Beatson, SA Comolli, JC Jakobsen, T Sargent, JL Bertrand, JJ West, J Klausen, M Waite, LL Kang, PJ Tolker-Nielsen, T Mattick, JS Engel, JN

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Publication Type:: Journal Article
Citation:: Molecular Microbiology, 2005, 55 (5), pp. 1357 - 1378
Issue Date:: 2005-03-01

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Field	Value	Language
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Beatson, SA	en_US
dc.contributor.author	Comolli, JC	en_US
dc.contributor.author	Jakobsen, T	en_US
dc.contributor.author	Sargent, JL	en_US
dc.contributor.author	Bertrand, JJ	en_US
dc.contributor.author	West, J	en_US
dc.contributor.author	Klausen, M	en_US
dc.contributor.author	Waite, LL	en_US
dc.contributor.author	Kang, PJ	en_US
dc.contributor.author	Tolker-Nielsen, T	en_US
dc.contributor.author	Mattick, JS	en_US
dc.contributor.author	Engel, JN	en_US
dc.date.issued	2005-03-01	en_US
dc.identifier.citation	Molecular Microbiology, 2005, 55 (5), pp. 1357 - 1378	en_US
dc.identifier.issn	0950-382X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8601
dc.description.abstract	Virulence of Pseudomonas aeruginosa involves the co-ordinate expression of a range of factors including type IV pili (tfp), the type III secretion system (TTSS) and quorum sensing. Tfp are required for twitching motility, efficient biofilm formation, and for adhesion and type III secretion (TTS)-mediated damage to mammalian cells. We describe a novel gene (fimL) that is required for tfp biogenesis and function, for TTS and for normal biofilm development in P. aeruginosa. The predicted product of fimL is homologous to the N-terminal domain of ChpA, except that its putative histidine and threonine phosphotransfer sites have been replaced with glutamine. fimL mutants resemble vfr mutants in many aspects including increased autolysis, reduced levels of surface-assembled tfp and diminished production of type III secreted effectors. Expression of vfr in trans can complement fimL mutants, vfr transcription and production is reduced in fimL mutants whereas cAMP levels are unaffected. Deletion and insertion mutants of fimL frequently revert to wild-type phenotypes suggesting that an extragenic suppressor mutation is able to overcome the loss of fimL. vfr transcription and production, as well as cAMP levels, are elevated in these revenants, while Pseudomonas quinolone signal (PQS) production is reduced. These results suggest that the site(s) of spontaneous mutation is in a gene(s) which lies upstream of vfr transcription, CAMP, production, and PQS synthesis. Our studies indicate that Vfr and FimL are components of intersecting pathways that control twitching motility, TTSS and autolysis in P. aeruginosa. © 2005 Blackwell Publishing Ltd.	en_US
dc.relation.ispartof	Molecular Microbiology	en_US
dc.relation.isbasedon	10.1111/j.1365-2958.2005.04479.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Fimbriae, Bacterial	en_US
dc.subject.mesh	Epithelial Cells	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Fimbriae Proteins	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Cyclic AMP Receptor Protein	en_US
dc.subject.mesh	Cyclic AMP	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Operon	en_US
dc.title	Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	55	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000227086300006	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	55	en_US

Abstract:

Virulence of Pseudomonas aeruginosa involves the co-ordinate expression of a range of factors including type IV pili (tfp), the type III secretion system (TTSS) and quorum sensing. Tfp are required for twitching motility, efficient biofilm formation, and for adhesion and type III secretion (TTS)-mediated damage to mammalian cells. We describe a novel gene (fimL) that is required for tfp biogenesis and function, for TTS and for normal biofilm development in P. aeruginosa. The predicted product of fimL is homologous to the N-terminal domain of ChpA, except that its putative histidine and threonine phosphotransfer sites have been replaced with glutamine. fimL mutants resemble vfr mutants in many aspects including increased autolysis, reduced levels of surface-assembled tfp and diminished production of type III secreted effectors. Expression of vfr in trans can complement fimL mutants, vfr transcription and production is reduced in fimL mutants whereas cAMP levels are unaffected. Deletion and insertion mutants of fimL frequently revert to wild-type phenotypes suggesting that an extragenic suppressor mutation is able to overcome the loss of fimL. vfr transcription and production, as well as cAMP levels, are elevated in these revenants, while Pseudomonas quinolone signal (PQS) production is reduced. These results suggest that the site(s) of spontaneous mutation is in a gene(s) which lies upstream of vfr transcription, CAMP, production, and PQS synthesis. Our studies indicate that Vfr and FimL are components of intersecting pathways that control twitching motility, TTSS and autolysis in P. aeruginosa. © 2005 Blackwell Publishing Ltd.

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http://hdl.handle.net/10453/8601