Improving protein solubility: The use of the Escherichia coli dihydrofolate reductase gene as a fusion reporter

Academic Press Inc Elsevier Science
Publication Type:
Journal Article
Protein Expression And Purification, 2006, 47 (1), pp. 258 - 263
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We have devised a strategy for screening mutant libraries for enzyme variants with enhanced solubility. The method is based on the observation that Escherichia coli can become insensitive to the antibiotic trimethoprim (TMP) if dihydrofolate reductase (D
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