Differential regulation of twitching motility and elastase production by Vfr in Pseudomonas aeruginosa

Beatson, SA; Whitchurch, CB; Sargent, JL; Levesque, RC; Mattick, JS

Differential regulation of twitching motility and elastase production by Vfr in Pseudomonas aeruginosa

Beatson, SA Whitchurch, CB

Sargent, JL Levesque, RC Mattick, JS

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2002, 184 (13), pp. 3605 - 3613
Issue Date:: 2002-06-24

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Field	Value	Language
dc.contributor.author	Beatson, SA	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Sargent, JL	en_US
dc.contributor.author	Levesque, RC	en_US
dc.contributor.author	Mattick, JS	en_US
dc.date.issued	2002-06-24	en_US
dc.identifier.citation	Journal of Bacteriology, 2002, 184 (13), pp. 3605 - 3613	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8612
dc.description.abstract	Vfr, a homolog of Escherichia coli cyclic AMP (cAMP) receptor protein, has been shown to regulate quorum sensing, exotoxin A production, and regA transcription in Pseudomonas aeruginosa. We identified a twitching motility-defective mutant that carries a transposon insertion in vfr and confirmed that vfr is required for twitching motility by construction of an independent allelic deletion-replacement mutant of vfr that exhibited the same phenotype, as well as by the restoration of normal twitching motility by complementation of these mutants with wild-type vfr. Vfr-null mutants exhibited severely reduced twitching motility with barely detectable levels of type IV pili, as well as loss of elastase production and altered pyocyanin production. We also identified reduced-twitching variants of quorum-sensing mutants (PAK lasI::Tc) with a spontaneous deletion in vfr (S. A. Beatson, C. B. Whitchurch, A. B. T. Semmler, and J. S. Mattick, J. Bacteriol., 184:3598-3604, 2002), the net result of which was the loss of five residues (EQERS) from the putative cAMP-binding pocket of Vfr. This allele (VfrΔEQERS) was capable of restoring elastase and pyocyanin production to wild-type levels in vfr-null mutants but not their defects in twitching motility. Furthermore, structural analysis of Vfr and VfrΔEQERS in relation to E. coli CRP suggests that Vfr is capable of binding both cAMP and cyclic GMP whereas VfrΔEQERS is only capable of responding to cAMP. We suggest that Vfr controls twitching motility and quorum sensing via independent pathways in response to these different signals, bound by the same cyclic nucleotide monophosphate-binding pocket.	en_US
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.184.13.3605-3613.2002	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Fimbriae, Bacterial	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Pyocyanine	en_US
dc.subject.mesh	Pancreatic Elastase	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Cyclic AMP Receptor Protein	en_US
dc.subject.mesh	Cyclic AMP	en_US
dc.subject.mesh	Cyclic GMP	en_US
dc.subject.mesh	Genetic Complementation Test	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Binding Sites	en_US
dc.subject.mesh	Protein Conformation	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Models, Molecular	en_US
dc.title	Differential regulation of twitching motility and elastase production by Vfr in Pseudomonas aeruginosa	en_US
dc.type	Journal Article
utslib.citation.volume	13	en_US
utslib.citation.volume	184	en_US
utslib.for	060501 Bacteriology	en_US
utslib.for	110801 Medical Bacteriology	en_US
utslib.for	110309 Infectious Diseases	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000176237400022	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	13	en_US
pubs.publication-status	Published	en_US
pubs.volume	184	en_US

Abstract:

Vfr, a homolog of Escherichia coli cyclic AMP (cAMP) receptor protein, has been shown to regulate quorum sensing, exotoxin A production, and regA transcription in Pseudomonas aeruginosa. We identified a twitching motility-defective mutant that carries a transposon insertion in vfr and confirmed that vfr is required for twitching motility by construction of an independent allelic deletion-replacement mutant of vfr that exhibited the same phenotype, as well as by the restoration of normal twitching motility by complementation of these mutants with wild-type vfr. Vfr-null mutants exhibited severely reduced twitching motility with barely detectable levels of type IV pili, as well as loss of elastase production and altered pyocyanin production. We also identified reduced-twitching variants of quorum-sensing mutants (PAK lasI::Tc) with a spontaneous deletion in vfr (S. A. Beatson, C. B. Whitchurch, A. B. T. Semmler, and J. S. Mattick, J. Bacteriol., 184:3598-3604, 2002), the net result of which was the loss of five residues (EQERS) from the putative cAMP-binding pocket of Vfr. This allele (VfrΔEQERS) was capable of restoring elastase and pyocyanin production to wild-type levels in vfr-null mutants but not their defects in twitching motility. Furthermore, structural analysis of Vfr and VfrΔEQERS in relation to E. coli CRP suggests that Vfr is capable of binding both cAMP and cyclic GMP whereas VfrΔEQERS is only capable of responding to cAMP. We suggest that Vfr controls twitching motility and quorum sensing via independent pathways in response to these different signals, bound by the same cyclic nucleotide monophosphate-binding pocket.

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http://hdl.handle.net/10453/8612