Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa

Beatson, SA; Whitchurch, CB; Semmler, ABT; Mattick, JS

Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa

Beatson, SA Whitchurch, CB

Semmler, ABT Mattick, JS

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2002, 184 (13), pp. 3598 - 3604
Issue Date:: 2002-06-24

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Field	Value	Language
dc.contributor.author	Beatson, SA	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Semmler, ABT	en_US
dc.contributor.author	Mattick, JS	en_US
dc.date.issued	2002-06-24	en_US
dc.identifier.citation	Journal of Bacteriology, 2002, 184 (13), pp. 3598 - 3604	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8617
dc.description.abstract	It has been reported that mutations in the quorum-sensing genes lasI and rhlI in Pseudomonas aeruginosa result in, among many other things, loss of twitching motility (A. Glessner, R. S. Smith, B. H. Iglewski, and J. B. Robinson, J. Bacteriol. 181:1623-1629, 1999). We constructed knockouts of lasI and rhlI and the corresponding regulatory genes lasR and rhlR and found no effect on twitching motility. However, twitching-defective variants accumulated during culturing of lasI and rhlI mutants. Further analysis showed that the stable twitching-defective variants of lasI and rhlI mutants had arisen as a consequence of secondary mutations in vfr and algR, respectively, both of which encode key regulators affecting a variety of phenotypes, including twitching motility. In addition, when grown in shaking broth culture, lasI and rhlI mutants, but not the wild-type parent, also accumulated unstable variants that lacked both twitching motility and swimming motility and appeared to be identical in phenotype to the S1 and S2 variants that were recently reported to occur at high frequencies in P. aeruginosa strains grown as a biofilm or in static broth culture (E. Deziel, Y. Comeau, and R. Villemur, J. Bacteriol. 183:1195-1204, 2001). These results indicate that mutations in one regulatory system may create distortions that select during subsequent culturing for compensatory mutations in other regulatory genes within the cellular network. This problem may have compromised some past studies of regulatory hierarchies controlled by quorum sensing and of bacterial regulatory systems in general.	en_US
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.184.13.3598-3604.2002	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Ligases	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Trans-Activators	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Cyclic AMP Receptor Protein	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Genes, Regulator	en_US
dc.title	Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa	en_US
dc.type	Journal Article
utslib.citation.volume	13	en_US
utslib.citation.volume	184	en_US
utslib.for	060501 Bacteriology	en_US
utslib.for	110801 Medical Bacteriology	en_US
utslib.for	110309 Infectious Diseases	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000176237400021	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	13	en_US
pubs.publication-status	Published	en_US
pubs.volume	184	en_US

Abstract:

It has been reported that mutations in the quorum-sensing genes lasI and rhlI in Pseudomonas aeruginosa result in, among many other things, loss of twitching motility (A. Glessner, R. S. Smith, B. H. Iglewski, and J. B. Robinson, J. Bacteriol. 181:1623-1629, 1999). We constructed knockouts of lasI and rhlI and the corresponding regulatory genes lasR and rhlR and found no effect on twitching motility. However, twitching-defective variants accumulated during culturing of lasI and rhlI mutants. Further analysis showed that the stable twitching-defective variants of lasI and rhlI mutants had arisen as a consequence of secondary mutations in vfr and algR, respectively, both of which encode key regulators affecting a variety of phenotypes, including twitching motility. In addition, when grown in shaking broth culture, lasI and rhlI mutants, but not the wild-type parent, also accumulated unstable variants that lacked both twitching motility and swimming motility and appeared to be identical in phenotype to the S1 and S2 variants that were recently reported to occur at high frequencies in P. aeruginosa strains grown as a biofilm or in static broth culture (E. Deziel, Y. Comeau, and R. Villemur, J. Bacteriol. 183:1195-1204, 2001). These results indicate that mutations in one regulatory system may create distortions that select during subsequent culturing for compensatory mutations in other regulatory genes within the cellular network. This problem may have compromised some past studies of regulatory hierarchies controlled by quorum sensing and of bacterial regulatory systems in general.

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http://hdl.handle.net/10453/8617