Diagnosis of human fasciolosis in the Gilan province of Northern Iran: Application of cathepsin L-ELISA

Rokni, MB; Massoud, J; O'Neill, SM; Parkinson, M; Dalton, JP

Diagnosis of human fasciolosis in the Gilan province of Northern Iran: Application of cathepsin L-ELISA

Rokni, MB Massoud, J O'Neill, SM Parkinson, M Dalton, JP

Permalink

Publication Type:: Journal Article
Citation:: Diagnostic Microbiology and Infectious Disease, 2002, 44 (2), pp. 175 - 179
Issue Date:: 2002-12-01

Closed Access

	Filename	Description	Size
	2006012454OK.pdf		144.1 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Rokni, MB	en_US
dc.contributor.author	Massoud, J	en_US
dc.contributor.author	O'Neill, SM	en_US
dc.contributor.author	Parkinson, M	en_US
dc.contributor.author	Dalton, JP	en_US
dc.date.issued	2002-12-01	en_US
dc.identifier.citation	Diagnostic Microbiology and Infectious Disease, 2002, 44 (2), pp. 175 - 179	en_US
dc.identifier.issn	0732-8893	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8629
dc.description.abstract	Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man, particularly in countries such as Bolivia, Peru and Egypt. Several outbreaks of this disease recently occurred in the Gilan province of Northern Iran and in 1999 alone over 10,000 individuals were infected. Our laboratory recently developed an enzyme linked immunosorbant assay (ELISA) test for diagnosing human fasciolosis in an endemic area of northern Bolivia. The assay was based on the detection of serum antibodies reactive with antigens secreted by the parasite. In the present report we examined the sensitivity and specificity of this ELISA to diagnose 176 patients residing in the Gilan province of Northern Iran. These individuals presented at health clinics with clinical symptoms of fasciolosis and were subsequently positively diagnosed by fecal analysis. The ELISA employed total molecules secreted by the parasites (excretory/secretory, ES, products) and a protease, termed cathepsin L1 (CL1), which was purified from this preparation, as antigen. In addition, the specificity of the assay was investigated using serum from Iranian individuals that were infected with hydatidosis, toxocariasis, amoebiasis, malaria and kalaazar. Using this assay, both CL1 and ES exhibited a sensitivity of 100% (all 176 patients tested positive) and a specificity of 100% and 98.9%, respectively. In conclusion, our standardized diagnostic ELISA for human fasciolosis based on the detection of IgG responses to parasite ES and CL1 would be a valuable tool to diagnosis human fasciolosis in Iran and could be employed in a large survey to determine the prevalence of the disease throughout this region. © 2002 Elsevier Science Inc. All rights reserved.	en_US
dc.relation.ispartof	Diagnostic Microbiology and Infectious Disease	en_US
dc.relation.isbasedon	10.1016/S0732-8893(02)00431-5	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Fasciola hepatica	en_US
dc.subject.mesh	Fascioliasis	en_US
dc.subject.mesh	Cathepsins	en_US
dc.subject.mesh	Cysteine Endopeptidases	en_US
dc.subject.mesh	Antibodies, Helminth	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Incidence	en_US
dc.subject.mesh	Risk Factors	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Disease Outbreaks	en_US
dc.subject.mesh	Age Distribution	en_US
dc.subject.mesh	Sex Distribution	en_US
dc.subject.mesh	Developing Countries	en_US
dc.subject.mesh	Adolescent	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Aged	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Child	en_US
dc.subject.mesh	Child, Preschool	en_US
dc.subject.mesh	Rural Population	en_US
dc.subject.mesh	Iran	en_US
dc.subject.mesh	Cathepsin L	en_US
dc.title	Diagnosis of human fasciolosis in the Gilan province of Northern Iran: Application of cathepsin L-ELISA	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	44	en_US
utslib.for	060502 Infectious Agents	en_US
utslib.for	060107 Enzymes	en_US
utslib.for	110803 Medical Parasitology	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	44	en_US

Abstract:

Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man, particularly in countries such as Bolivia, Peru and Egypt. Several outbreaks of this disease recently occurred in the Gilan province of Northern Iran and in 1999 alone over 10,000 individuals were infected. Our laboratory recently developed an enzyme linked immunosorbant assay (ELISA) test for diagnosing human fasciolosis in an endemic area of northern Bolivia. The assay was based on the detection of serum antibodies reactive with antigens secreted by the parasite. In the present report we examined the sensitivity and specificity of this ELISA to diagnose 176 patients residing in the Gilan province of Northern Iran. These individuals presented at health clinics with clinical symptoms of fasciolosis and were subsequently positively diagnosed by fecal analysis. The ELISA employed total molecules secreted by the parasites (excretory/secretory, ES, products) and a protease, termed cathepsin L1 (CL1), which was purified from this preparation, as antigen. In addition, the specificity of the assay was investigated using serum from Iranian individuals that were infected with hydatidosis, toxocariasis, amoebiasis, malaria and kalaazar. Using this assay, both CL1 and ES exhibited a sensitivity of 100% (all 176 patients tested positive) and a specificity of 100% and 98.9%, respectively. In conclusion, our standardized diagnostic ELISA for human fasciolosis based on the detection of IgG responses to parasite ES and CL1 would be a valuable tool to diagnosis human fasciolosis in Iran and could be employed in a large survey to determine the prevalence of the disease throughout this region. © 2002 Elsevier Science Inc. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/8629