An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection

McArthur, JD; West, NP; Cole, JN; Jungnitz, H; Guzmán, CA; Chin, J; Lehrbach, PR; Djordjevic, SP; Walker, MJ

An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection

McArthur, JD West, NP Cole, JN Jungnitz, H Guzmán, CA Chin, J Lehrbach, PR Djordjevic, SP

Walker, MJ

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Publication Type:: Journal Article
Citation:: FEMS Microbiology Letters, 2003, 221 (1), pp. 7 - 16
Issue Date:: 2003-04-11

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Field	Value	Language
dc.contributor.author	McArthur, JD	en_US
dc.contributor.author	West, NP	en_US
dc.contributor.author	Cole, JN	en_US
dc.contributor.author	Jungnitz, H	en_US
dc.contributor.author	Guzmán, CA	en_US
dc.contributor.author	Chin, J	en_US
dc.contributor.author	Lehrbach, PR	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Walker, MJ	en_US
dc.date.issued	2003-04-11	en_US
dc.identifier.citation	FEMS Microbiology Letters, 2003, 221 (1), pp. 7 - 16	en_US
dc.identifier.issn	0378-1097	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8634
dc.description.abstract	We have constructed an aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica, harbouring mutations in aroA and trpE to investigate the use of such a strain as a live-attenuated vaccine. B. bronchiseptica aroA trpE was unable to grow in minimal medium without aromatic supplementation. Compared to the parental wild-type strain, the mutant displayed significantly reduced abilities to invade and survive within the mouse macrophage-like cell line J774A.1 in vitro and in the murine respiratory tract following experimental intranasal infection. Mice vaccinated with B. bronchiseptica aroA trpE displayed significant dose-dependent increases in B. bronchiseptica-specific antibody responses, and exhibited increases in the number of B. bronchiseptica-reactive spleen cells in lymphoproliferation assays. Immunised animals were protected against lung colonisation after challenge with the wild-type parental strain. With such a broad host range displayed by B. bronchiseptica, the attenuated strain constructed in this study may not only be used for the prevention of B. bronchiseptica-associated disease, but also for the potential delivery of heterologous antigen. © 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.	en_US
dc.relation.ispartof	FEMS Microbiology Letters	en_US
dc.relation.isbasedon	10.1016/S0378-1097(03)00162-9	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred BALB C	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Bordetella bronchiseptica	en_US
dc.subject.mesh	Bordetella Infections	en_US
dc.subject.mesh	Disease Models, Animal	en_US
dc.subject.mesh	Anthranilate Synthase	en_US
dc.subject.mesh	Alkyl and Aryl Transferases	en_US
dc.subject.mesh	Amino Acids, Aromatic	en_US
dc.subject.mesh	Bacterial Vaccines	en_US
dc.subject.mesh	Vaccines, Attenuated	en_US
dc.subject.mesh	Antibodies, Bacterial	en_US
dc.subject.mesh	Vaccination	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Lymphocyte Activation	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Operon	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	3-Phosphoshikimate 1-Carboxyvinyltransferase	en_US
dc.title	An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	221	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	221	en_US

Abstract:

We have constructed an aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica, harbouring mutations in aroA and trpE to investigate the use of such a strain as a live-attenuated vaccine. B. bronchiseptica aroA trpE was unable to grow in minimal medium without aromatic supplementation. Compared to the parental wild-type strain, the mutant displayed significantly reduced abilities to invade and survive within the mouse macrophage-like cell line J774A.1 in vitro and in the murine respiratory tract following experimental intranasal infection. Mice vaccinated with B. bronchiseptica aroA trpE displayed significant dose-dependent increases in B. bronchiseptica-specific antibody responses, and exhibited increases in the number of B. bronchiseptica-reactive spleen cells in lymphoproliferation assays. Immunised animals were protected against lung colonisation after challenge with the wild-type parental strain. With such a broad host range displayed by B. bronchiseptica, the attenuated strain constructed in this study may not only be used for the prevention of B. bronchiseptica-associated disease, but also for the potential delivery of heterologous antigen. © 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/8634