Integrons found in different locations have identical 5' ends but variable 3' ends.

Hall, RM; Brown, HJ; Brookes, DE; Stokes, HW

Integrons found in different locations have identical 5' ends but variable 3' ends.

Hall, RM Brown, HJ Brookes, DE Stokes, HW

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Publication Type:: Journal Article
Citation:: J Bacteriol, 1994, 176 (20), pp. 6286 - 6294
Issue Date:: 1994-10

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Field	Value	Language
dc.contributor.author	Hall, RM	en_US
dc.contributor.author	Brown, HJ	en_US
dc.contributor.author	Brookes, DE	en_US
dc.contributor.author	Stokes, HW	en_US
dc.date.issued	1994-10	en_US
dc.identifier.citation	J Bacteriol, 1994, 176 (20), pp. 6286 - 6294	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8645
dc.description.abstract	The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (pSCH884), and In0 (pVS1). However, the extent of the 3'-conserved segment differs in each integron. The sequences of In2 and In0 diverge first from the conserved sequence, and their divergence point corresponds to the 3'-conserved segment endpoint defined previously (H.W. Stokes and R.M. Hall, Mol. Microbiol. 3:1669-1683, 1989), which now represents the endpoint of a 359-base deletion in In0 and In2. The sequence identity in In3, In1, In4, and In5 extends beyond this point, but each sequence diverges from the conserved sequence at a different point within a short region. Insertions of IS6100 were identified adjacent to the end of the conserved region in In1 and 123 bases beyond the divergence point of In4. These 123 bases are identical to the sequence found at the mer end of the 11.2-kb insertion in Tn21 but are inverted. In5 and In0 are bounded by the same 25-base inverted repeat that bounds the 11.2-kb insert in Tn21, and this insert now corresponds to In2. However, while In0, In2, and In5 have features characteristic of transposable elements, differences in the structures of these three integrons and the absence of evidence of mobility currently preclude the identification of all of the sequences associated with a functional transposon of this type.	en_US
dc.language	eng	en_US
dc.relation.ispartof	J Bacteriol	en_US
dc.relation.isbasedon	10.1128/jb.176.20.6286-6294.1994	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Sulfonamides	en_US
dc.subject.mesh	DNA Transposable Elements	en_US
dc.subject.mesh	Restriction Mapping	en_US
dc.subject.mesh	Cloning, Molecular	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Drug Resistance, Microbial	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Repetitive Sequences, Nucleic Acid	en_US
dc.subject.mesh	Conserved Sequence	en_US
dc.subject.mesh	Sequence Homology, Nucleic Acid	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Integrons found in different locations have identical 5' ends but variable 3' ends.	en_US
dc.type	Journal Article
utslib.citation.volume	20	en_US
utslib.citation.volume	176	en_US
utslib.location.activity	United States	en_US
utslib.for	060503 Microbial Genetics	en_US
utslib.for	060406 Genetic Immunology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	20	en_US
pubs.publication-status	Published	en_US
pubs.volume	176	en_US

Abstract:

The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (pSCH884), and In0 (pVS1). However, the extent of the 3'-conserved segment differs in each integron. The sequences of In2 and In0 diverge first from the conserved sequence, and their divergence point corresponds to the 3'-conserved segment endpoint defined previously (H.W. Stokes and R.M. Hall, Mol. Microbiol. 3:1669-1683, 1989), which now represents the endpoint of a 359-base deletion in In0 and In2. The sequence identity in In3, In1, In4, and In5 extends beyond this point, but each sequence diverges from the conserved sequence at a different point within a short region. Insertions of IS6100 were identified adjacent to the end of the conserved region in In1 and 123 bases beyond the divergence point of In4. These 123 bases are identical to the sequence found at the mer end of the 11.2-kb insertion in Tn21 but are inverted. In5 and In0 are bounded by the same 25-base inverted repeat that bounds the 11.2-kb insert in Tn21, and this insert now corresponds to In2. However, while In0, In2, and In5 have features characteristic of transposable elements, differences in the structures of these three integrons and the absence of evidence of mobility currently preclude the identification of all of the sequences associated with a functional transposon of this type.

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http://hdl.handle.net/10453/8645