Searching for new markers of endogenous steroid administration in athletes: looking outside the metabolic box

Publisher:
Elsevier Sci Ireland Ltd
Publication Type:
Journal Article
Citation:
Forensic Science International, 2004, 143 (2), pp. 103 - 114
Issue Date:
2004-01
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A simple means of detecting the abuse of steroids that also occur naturally is a problem facing doping control laboratories. Specific markers are required to allow the detection of the administration of these steroids. These markers are commonly measured using a set of data obtained from the screening of samples by gas chromatographymass spectrometry (GCMS). Doping control laboratories further need to confirm identified abuse using techniques such as gas chromatographycombustionisotope ratio mass spectrometry (GCCIRMS). An interesting urinary species was found while following the pharmacokinetics and changes to the steroid profile from single and multiple oral doses of the International Olympic Committee/World Anti Doping Agency (IOC/WADA) prohibited substance, dehydroepiandrosterone (DHEA). The urine samples collected from the administration studies were subject to GCMS and GCCIRMS steroid analysis following cleanup by solid phase extraction techniques. A useful urinary product of DHEA administration was detected in the urine samples from each of the administration studies and was identified by GCMS experiments to be 3?,5-cyclo-5?-androstan-6?-ol-17-one (3?,5-cyclo). This compound occurs naturally but the concentrations of 3?,5-cyclo were elevated following both the single DHEA administration (up to 385ng/mL) and multiple DHEA administrations (up to 1240ng/mL), in relation to those observed prior to these administrations (70 and 80ng/mL, respectively).
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