Comparative study of selenium requirements of three phytoplankton species: Gymnodinium catenatum, Alexandrium minutum (Dinophyta) and Chaetoceros cf. tenuissimus (Bacillariophyta)

Doblin, MA; Blackburn, SI; Hallegraeff, GM

Comparative study of selenium requirements of three phytoplankton species: Gymnodinium catenatum, Alexandrium minutum (Dinophyta) and Chaetoceros cf. tenuissimus (Bacillariophyta)

Doblin, MA

Blackburn, SI Hallegraeff, GM

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Publication Type:: Journal Article
Citation:: Journal of Plankton Research, 1999, 21 (6), pp. 1153 - 1169
Issue Date:: 1999-01-01

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Field	Value	Language
dc.contributor.author	Doblin, MA https://orcid.org/0000-0001-8750-3433	en_US
dc.contributor.author	Blackburn, SI	en_US
dc.contributor.author	Hallegraeff, GM https://orcid.org/0000-0001-8464-7343	en_US
dc.date.issued	1999-01-01	en_US
dc.identifier.citation	Journal of Plankton Research, 1999, 21 (6), pp. 1153 - 1169	en_US
dc.identifier.issn	0142-7873	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8681
dc.description.abstract	This study investigated the selenium (Se) requirements of three phytoplankton species which commonly bloom in southern Australian estuaries. The present study showed that the toxic dinoflagellate Gymnodinium catenatum Graham had an obligate requirement for Se (IV) in culture. After two transfers (~4 weeks ≃ 7 generations) in Se-deficient seawater medium, this phytoplankton species exhibited a decline in growth rate (25%) and biomass yield (90%), while complete cessation of cell division occurred under prolonged (8 weeks ≃ 12 generations) Se starvation. Addition of 10-9-10-7 M H2SeO3 to nutrient-enriched seawater medium resulted in increased G.catenatum growth and biomass yields in direct proportion to the Se concentrations offered. In contrast to G.catenatum, Se limitation was observed in the dinoflagellate Alexandrium minutum Halim after four transfers (5 weeks ≃ 20 generations) in Se-deficient medium. Exponential growth rates of A.minutum decreased slightly (5-10%) when Se was not supplied, but biomass yields decreased as much as 80-90%. The diatom Chaetoceros cf. tenuissimus Meunier showed no evidence of Se limitation even after eight transfers (8 weeks; >60 generations) in Se-deficient medium. Variations in growth rates and biomass yields between transfers provide valuable information about the relative potential for Se limitation in the three species studied. In addition, differences in Se requirement between these bloom-forming phytoplankton species suggest that this micronutrient may play a role in structuring phytoplankton communities in southern Australian waters.	en_US
dc.relation.ispartof	Journal of Plankton Research	en_US
dc.relation.isbasedon	10.1093/plankt/21.6.1153	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	Comparative study of selenium requirements of three phytoplankton species: Gymnodinium catenatum, Alexandrium minutum (Dinophyta) and Chaetoceros cf. tenuissimus (Bacillariophyta)	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	21	en_US
utslib.for	060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)	en_US
utslib.for	039901 Environmental Chemistry (incl. Atmospheric Chemistry)	en_US
utslib.for	040501 Biological Oceanography	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0704 Fisheries Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	21	en_US

Abstract:

This study investigated the selenium (Se) requirements of three phytoplankton species which commonly bloom in southern Australian estuaries. The present study showed that the toxic dinoflagellate Gymnodinium catenatum Graham had an obligate requirement for Se (IV) in culture. After two transfers (~4 weeks ≃ 7 generations) in Se-deficient seawater medium, this phytoplankton species exhibited a decline in growth rate (25%) and biomass yield (90%), while complete cessation of cell division occurred under prolonged (8 weeks ≃ 12 generations) Se starvation. Addition of 10-9-10-7 M H2SeO3 to nutrient-enriched seawater medium resulted in increased G.catenatum growth and biomass yields in direct proportion to the Se concentrations offered. In contrast to G.catenatum, Se limitation was observed in the dinoflagellate Alexandrium minutum Halim after four transfers (5 weeks ≃ 20 generations) in Se-deficient medium. Exponential growth rates of A.minutum decreased slightly (5-10%) when Se was not supplied, but biomass yields decreased as much as 80-90%. The diatom Chaetoceros cf. tenuissimus Meunier showed no evidence of Se limitation even after eight transfers (8 weeks; >60 generations) in Se-deficient medium. Variations in growth rates and biomass yields between transfers provide valuable information about the relative potential for Se limitation in the three species studied. In addition, differences in Se requirement between these bloom-forming phytoplankton species suggest that this micronutrient may play a role in structuring phytoplankton communities in southern Australian waters.

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http://hdl.handle.net/10453/8681