A second generation multiplex PCR for typing strains of Neospora caninum using six DNA targets

Al-Qassab, S; Reichel, MP; Ellis, J

A second generation multiplex PCR for typing strains of Neospora caninum using six DNA targets

Al-Qassab, S Reichel, MP

Ellis, J

Permalink

Publication Type:: Journal Article
Citation:: Molecular and Cellular Probes, 2010, 24 (1), pp. 20 - 26
Issue Date:: 2010-02-01

Closed Access

	Filename	Description	Size
	2008008313OK.pdf		433.12 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Al-Qassab, S	en_US
dc.contributor.author	Reichel, MP https://orcid.org/0000-0002-9792-8336	en_US
dc.contributor.author	Ellis, J https://orcid.org/0000-0001-7328-4831	en_US
dc.date.available	2009-08-10	en_US
dc.date.issued	2010-02-01	en_US
dc.identifier.citation	Molecular and Cellular Probes, 2010, 24 (1), pp. 20 - 26	en_US
dc.identifier.issn	0890-8508	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8720
dc.description.abstract	Genetic diversity of Neospora caninum was investigated through a study of repetitive sequences found in the genome of this species. Twenty different loci were studied, and three were identified that varied in repeat content amongst isolates. No relationship was found between the copy number of repetitive sequences present and host type or geographical location from which the isolates were derived. A multiplex PCR assay was developed for multilocus-strain typing using three microsatellites and three minisatellites, based on the polymorphisms found in the repetitive sequences. This study therefore extends knowledge on the repetitive sequences found in the N. caninum genome and the diversity found within the species. It also provides a second generation multiplex assay that can be used to study the biology of N. caninum. In addition, this study included Neospora hughesi (along with other closely related apicomplexans) as controls. The present study shows N. hughesi to be quite distinct from N. caninum in these repetitive sequences, thereby potentially providing a new approach for the differentiation of these two taxa. © 2009 Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Molecular and Cellular Probes	en_US
dc.relation.isbasedon	10.1016/j.mcp.2009.08.002	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Neospora	en_US
dc.subject.mesh	DNA, Protozoan	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Microsatellite Repeats	en_US
dc.title	A second generation multiplex PCR for typing strains of Neospora caninum using six DNA targets	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	24	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	24	en_US

Abstract:

Genetic diversity of Neospora caninum was investigated through a study of repetitive sequences found in the genome of this species. Twenty different loci were studied, and three were identified that varied in repeat content amongst isolates. No relationship was found between the copy number of repetitive sequences present and host type or geographical location from which the isolates were derived. A multiplex PCR assay was developed for multilocus-strain typing using three microsatellites and three minisatellites, based on the polymorphisms found in the repetitive sequences. This study therefore extends knowledge on the repetitive sequences found in the N. caninum genome and the diversity found within the species. It also provides a second generation multiplex assay that can be used to study the biology of N. caninum. In addition, this study included Neospora hughesi (along with other closely related apicomplexans) as controls. The present study shows N. hughesi to be quite distinct from N. caninum in these repetitive sequences, thereby potentially providing a new approach for the differentiation of these two taxa. © 2009 Elsevier Ltd. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/8720