Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae.

Publisher:
Nature Publishing Group
Publication Type:
Journal Article
Citation:
Nature Communications, 2017, 8
Issue Date:
2017-03-08
Full metadata record
Files in This Item:
Filename Description Size
ncomms14665.pdfPublished Version5.64 MB
Adobe PDF
DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.
Please use this identifier to cite or link to this item: