Efficiency of recombination reactions catalyzed by class 1 integron integrase IntI1.
- Publication Type:
- Journal Article
- Citation:
- J Bacteriol, 2001, 183 (8), pp. 2535 - 2542
- Issue Date:
- 2001-04
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 | 2008005539OK.pdf | 114.43 kB |
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The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between attI1 and a 59-be, or between two attI1 sites, were compared. Recombination events involving two attI1 sites were significantly less efficient than the reactions in which a 59-be participated, and the attI1 x 59-be reaction was generally preferred over the 59-be x 59-be reaction. Recombination of attI1 with secondary sites was less efficient than the 59-be x secondary site reaction.
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