Assessment of microzooplankton grazing on Heterosigma akashiwo using a species- specific approach combining quantitative real-time PCR (QPCR) and dilution methods

Demir, E; Coyne, KJ; Doblin, MA; Handy, SM; Hutchins, DA

Assessment of microzooplankton grazing on Heterosigma akashiwo using a species- specific approach combining quantitative real-time PCR (QPCR) and dilution methods

Demir, E Coyne, KJ Doblin, MA

Handy, SM Hutchins, DA

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Publication Type:: Journal Article
Citation:: Microbial Ecology, 2008, 55 (4), pp. 583 - 594
Issue Date:: 2008-01-01

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Field	Value	Language
dc.contributor.author	Demir, E	en_US
dc.contributor.author	Coyne, KJ	en_US
dc.contributor.author	Doblin, MA https://orcid.org/0000-0001-8750-3433	en_US
dc.contributor.author	Handy, SM	en_US
dc.contributor.author	Hutchins, DA	en_US
dc.date.available	2007-04-17	en_US
dc.date.issued	2008-01-01	en_US
dc.identifier.citation	Microbial Ecology, 2008, 55 (4), pp. 583 - 594	en_US
dc.identifier.issn	0095-3628	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8744
dc.description.abstract	Delaware's Inland Bays (DIB) are subject to numerous mixed blooms of harmful raphidophytes each year, and Heterosigma akashiwo is one of the consistently occurring species. Often, Chattonella subsalsa, C. cf. verruculosa, and Fibrocapsa japonica co-occur with H. akashiwo, indicating a dynamic consortium of raphidophyte species. In this study, microzooplankton grazing pressure was assessed as a top-down control mechanism on H. akashiwo populations in mixed communities. Quantitative real-time polymerase chain reaction (QPCR) with species-specific primers and probes were used in conjunction with the dilution method to assess grazing pressure on H. akashiwo and other raphidophytes. As a comparison, we measured changes in chlorophyll a (chl a) to determine whole community growth and mortality caused by grazing. We detected grazing on H. akashiwo using QPCR in samples where chl a analyses indicated little or no grazing on the total phytoplankton community. Overall, specific microzooplankton grazing pressure on H. akashiwo ranged from 0.88 to 1.88 day-1 at various sites. Experiments conducted on larger sympatric raphidophytes (C. subsalsa, C. cf. verruculosa and F. japonica) demonstrated no significant microzooplankton grazing on these species. Grazing pressure on H. akashiwo may provide a competitive advantage to other raphidophytes such as Chattonella spp. that are too large to be consumed at high rates by microzooplankton and help to shape the dynamics of this harmful algal bloom consortium. Our results show that QPCR can be used in conjunction with the dilution method for evaluation of microzooplankton grazing pressure on specific phytoplankton species within a mixed community. © 2007 Springer Science+Business Media, LLC.	en_US
dc.relation.ispartof	Microbial Ecology	en_US
dc.relation.isbasedon	10.1007/s00248-007-9263-9	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Zooplankton	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	DNA, Algal	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Population Dynamics	en_US
dc.subject.mesh	Eutrophication	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Delaware	en_US
dc.subject.mesh	Chlorophyll A	en_US
dc.title	Assessment of microzooplankton grazing on Heterosigma akashiwo using a species- specific approach combining quantitative real-time PCR (QPCR) and dilution methods	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	55	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0503 Soil Sciences	en_US
utslib.for	0605 Microbiology	en_US
dc.location.activity	ISI:000255411900003	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	55	en_US

Abstract:

Delaware's Inland Bays (DIB) are subject to numerous mixed blooms of harmful raphidophytes each year, and Heterosigma akashiwo is one of the consistently occurring species. Often, Chattonella subsalsa, C. cf. verruculosa, and Fibrocapsa japonica co-occur with H. akashiwo, indicating a dynamic consortium of raphidophyte species. In this study, microzooplankton grazing pressure was assessed as a top-down control mechanism on H. akashiwo populations in mixed communities. Quantitative real-time polymerase chain reaction (QPCR) with species-specific primers and probes were used in conjunction with the dilution method to assess grazing pressure on H. akashiwo and other raphidophytes. As a comparison, we measured changes in chlorophyll a (chl a) to determine whole community growth and mortality caused by grazing. We detected grazing on H. akashiwo using QPCR in samples where chl a analyses indicated little or no grazing on the total phytoplankton community. Overall, specific microzooplankton grazing pressure on H. akashiwo ranged from 0.88 to 1.88 day-1 at various sites. Experiments conducted on larger sympatric raphidophytes (C. subsalsa, C. cf. verruculosa and F. japonica) demonstrated no significant microzooplankton grazing on these species. Grazing pressure on H. akashiwo may provide a competitive advantage to other raphidophytes such as Chattonella spp. that are too large to be consumed at high rates by microzooplankton and help to shape the dynamics of this harmful algal bloom consortium. Our results show that QPCR can be used in conjunction with the dilution method for evaluation of microzooplankton grazing pressure on specific phytoplankton species within a mixed community. © 2007 Springer Science+Business Media, LLC.

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http://hdl.handle.net/10453/8744