The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR

McKee, BA; Djordjevic, SP; Goodman, RD; Hornitzky, MA

The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR

McKee, BA Djordjevic, SP

Goodman, RD Hornitzky, MA

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Publication Type:: Journal Article
Citation:: Apidologie, 2003, 34 (1), pp. 19 - 27
Issue Date:: 2003-01-01

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Field	Value	Language
dc.contributor.author	McKee, BA	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Goodman, RD	en_US
dc.contributor.author	Hornitzky, MA	en_US
dc.date.issued	2003-01-01	en_US
dc.identifier.citation	Apidologie, 2003, 34 (1), pp. 19 - 27	en_US
dc.identifier.issn	0044-8435	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8747
dc.description.abstract	A hemi-nested polymerase chain reaction (PCR) was further developed for the detection of Melissococcus pluton in adult bees and honey bee products. A chloroform:isoamyl alcohol DNA extraction method was used to provide template from 154 samples of adult bee tissues, honey, pollen, whole larvae and comb cells. All 36 honey bee samples tested from a diseased colony were shown to contain M. pluton and sub-clinical infections were detected in adult bee tissues, larvae and honey (49/98; 50.0%) collected from all 9 healthy colonies from areas where EFB was endemic. All 20 adult bee tissue samples from a healthy colony from Western Australia where EFB has never been reported were negative. Of 80 bulk honey samples from six Australian states, 55 of 80 (68.8%) samples were shown to contain M. pluton whereas culture techniques detected M. pluton in 22 of 80 (27.5%) of these samples. M. pluton was detected in honey from all Australian states except Western Australia.	en_US
dc.relation.ispartof	Apidologie	en_US
dc.relation.isbasedon	10.1051/apido:2002047	en_US
dc.subject.classification	Entomology	en_US
dc.title	The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	34	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	34	en_US

Abstract:

A hemi-nested polymerase chain reaction (PCR) was further developed for the detection of Melissococcus pluton in adult bees and honey bee products. A chloroform:isoamyl alcohol DNA extraction method was used to provide template from 154 samples of adult bee tissues, honey, pollen, whole larvae and comb cells. All 36 honey bee samples tested from a diseased colony were shown to contain M. pluton and sub-clinical infections were detected in adult bee tissues, larvae and honey (49/98; 50.0%) collected from all 9 healthy colonies from areas where EFB was endemic. All 20 adult bee tissue samples from a healthy colony from Western Australia where EFB has never been reported were negative. Of 80 bulk honey samples from six Australian states, 55 of 80 (68.8%) samples were shown to contain M. pluton whereas culture techniques detected M. pluton in 22 of 80 (27.5%) of these samples. M. pluton was detected in honey from all Australian states except Western Australia.

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http://hdl.handle.net/10453/8747