New enzymes from environmental cassette arrays: Functional attributes of a phosphotransferase and an RNA-methyltransferase

Publisher:
Cold Spring Harbor Lab Press
Publication Type:
Journal Article
Citation:
Protein Science, 2004, 13 (6), pp. 1651 - 1659
Issue Date:
2004-01
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By targeting gene cassettes by polymerase chain reaction (PCR) directly from environmentally derived DNA, we are able to amplify entire open reading frames (ORFs) independently of prior sequence knowledge. Approximately 10% of the mobile genes recovered
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