Twitching motility is essential for virulence in Dichelobacter nodosus

Han, X; Kennan, RM; Davies, JK; Reddacliff, LA; Dhungyel, OP; Whittington, RJ; Turnbull, L; Whitchurch, CB; Rood, JI

Twitching motility is essential for virulence in Dichelobacter nodosus

Han, X Kennan, RM Davies, JK Reddacliff, LA Dhungyel, OP Whittington, RJ Turnbull, L

Whitchurch, CB

Rood, JI

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2008, 190 (9), pp. 3323 - 3335
Issue Date:: 2008-05-01

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Field	Value	Language
dc.contributor.author	Han, X	en_US
dc.contributor.author	Kennan, RM	en_US
dc.contributor.author	Davies, JK	en_US
dc.contributor.author	Reddacliff, LA	en_US
dc.contributor.author	Dhungyel, OP	en_US
dc.contributor.author	Whittington, RJ	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Rood, JI	en_US
dc.date.issued	2008-05-01	en_US
dc.identifier.citation	Journal of Bacteriology, 2008, 190 (9), pp. 3323 - 3335	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8776
dc.description.abstract	Type IV fimbriae are essential virulence factors of Dichelobacter nodosus, the principal causative agent of ovine foot rot. The fimA fimbrial subunit gene is required for virulence, but fimA mutants exhibit several phenotypic changes and it is not certain if the effects on virulence result from the loss of type IV fimbria-mediated twitching motility, cell adherence, or reduced protease secretion. We showed that mutation of either the pilT or pilU gene eliminated the ability to carry out twitching motility. However, the pilT mutants displayed decreased adhesion to epithelial cells and reduced protease secretion, whereas the pilU mutants had wild-type levels of extracellular protease secretion and adherence. These data provided evidence that PilT is required for the type IV fimbria-dependent protease secretion pathway in D. nodosus. It was postulated that sufficient fimbrial retraction must occur in the pilU mutants to allow protease secretion, but not twitching motility, to take place. Although no cell movement was detected in a pilU mutant of D. nodosus, aberrant motion was detected in an equivalent mutant of Pseudomonas aeruginosa. These observations explain how in D. nodosus protease secretion can occur in a pilU mutant but not in a pilT mutant. In addition, virulence studies with sheep showed that both the pilT and pilU mutants were avirulent, providing evidence that mutation of the type IV fimbrial system affects virulence by eliminating twitching motility, not by altering cell adherence or protease secretion. Copyright © 2008, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.01807-07	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Fimbriae, Bacterial	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Dichelobacter nodosus	en_US
dc.subject.mesh	Foot Rot	en_US
dc.subject.mesh	Sheep Diseases	en_US
dc.subject.mesh	Peptide Hydrolases	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Fimbriae Proteins	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Protein Transport	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Molecular Motor Proteins	en_US
dc.subject.mesh	Adenosine Triphosphatases	en_US
dc.title	Twitching motility is essential for virulence in Dichelobacter nodosus	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	190	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000255202100026	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	190	en_US

Abstract:

Type IV fimbriae are essential virulence factors of Dichelobacter nodosus, the principal causative agent of ovine foot rot. The fimA fimbrial subunit gene is required for virulence, but fimA mutants exhibit several phenotypic changes and it is not certain if the effects on virulence result from the loss of type IV fimbria-mediated twitching motility, cell adherence, or reduced protease secretion. We showed that mutation of either the pilT or pilU gene eliminated the ability to carry out twitching motility. However, the pilT mutants displayed decreased adhesion to epithelial cells and reduced protease secretion, whereas the pilU mutants had wild-type levels of extracellular protease secretion and adherence. These data provided evidence that PilT is required for the type IV fimbria-dependent protease secretion pathway in D. nodosus. It was postulated that sufficient fimbrial retraction must occur in the pilU mutants to allow protease secretion, but not twitching motility, to take place. Although no cell movement was detected in a pilU mutant of D. nodosus, aberrant motion was detected in an equivalent mutant of Pseudomonas aeruginosa. These observations explain how in D. nodosus protease secretion can occur in a pilU mutant but not in a pilT mutant. In addition, virulence studies with sheep showed that both the pilT and pilU mutants were avirulent, providing evidence that mutation of the type IV fimbrial system affects virulence by eliminating twitching motility, not by altering cell adherence or protease secretion. Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/8776