Genetic and life-history trait variation of the amphipod Melita plumulosa from polluted and unpolluted waterways in eastern Australia

Chung, PP; Hyne, RV; Mann, RM; Ballard, JWO

Genetic and life-history trait variation of the amphipod Melita plumulosa from polluted and unpolluted waterways in eastern Australia

Chung, PP Hyne, RV Mann, RM

Ballard, JWO

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Publication Type:: Journal Article
Citation:: Science of the Total Environment, 2008, 403 (1-3), pp. 222 - 229
Issue Date:: 2008-09-15

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Field	Value	Language
dc.contributor.author	Chung, PP	en_US
dc.contributor.author	Hyne, RV	en_US
dc.contributor.author	Mann, RM https://orcid.org/0000-0001-7696-0751	en_US
dc.contributor.author	Ballard, JWO	en_US
dc.date.available	2008-05-28	en_US
dc.date.issued	2008-09-15	en_US
dc.identifier.citation	Science of the Total Environment, 2008, 403 (1-3), pp. 222 - 229	en_US
dc.identifier.issn	0048-9697	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8780
dc.description.abstract	To monitor genetic diversity and environmental contamination in eastern Australia, toxicity studies have employed the sensitive benthic amphipod Melita plumulosa. The goal of this study was to examine the genetic and life-history variability of natural populations of M. plumulosa from the Parramatta (polluted) and Hawkesbury (unpolluted) Rivers. The underlying genetics of the populations in these distinct waterways was examined at one mitochondrial (cytochrome c oxidase subunit I (COI)) and one nuclear (ribosomal internal transcribed spacer region 1 (ITS1)) locus. Seven unique haplotypes for COI were found amongst animals from the Parramatta River, while animals from the Hawkesbury River showed a complete absence of genetic variation at this locus. At ITS1 a total of two sequence variants were found amongst Parramatta River amphipods and three sequence variants among Hawkesbury River animals, with no common variants across the two river systems. To establish whether genetic differences were associated with organismal responses to toxicant exposure, two life-history trait variables (female head length as an estimator of amphipod size and female fecundity) were analyzed. Life-history trait analyses showed that females from the Hawkesbury River were significantly larger and more fecund. These data have critical implications for toxicity tests, the use of laboratory cultures for testing purposes, and environmental contamination in Sydney Harbor. © 2008 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Science of the Total Environment	en_US
dc.relation.isbasedon	10.1016/j.scitotenv.2008.05.038	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Head	en_US
dc.subject.mesh	Cell Nucleus	en_US
dc.subject.mesh	Mitochondria	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Amphipoda	en_US
dc.subject.mesh	Electron Transport Complex IV	en_US
dc.subject.mesh	DNA, Mitochondrial	en_US
dc.subject.mesh	DNA, Ribosomal Spacer	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Fresh Water	en_US
dc.subject.mesh	Environmental Monitoring	en_US
dc.subject.mesh	Water Pollution	en_US
dc.subject.mesh	Life Cycle Stages	en_US
dc.subject.mesh	Fertility	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Genetic Variation	en_US
dc.title	Genetic and life-history trait variation of the amphipod Melita plumulosa from polluted and unpolluted waterways in eastern Australia	en_US
dc.type	Journal Article
utslib.citation.volume	1-3	en_US
utslib.citation.volume	403	en_US
utslib.for	0602 Ecology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1-3	en_US
pubs.publication-status	Published	en_US
pubs.volume	403	en_US

Abstract:

To monitor genetic diversity and environmental contamination in eastern Australia, toxicity studies have employed the sensitive benthic amphipod Melita plumulosa. The goal of this study was to examine the genetic and life-history variability of natural populations of M. plumulosa from the Parramatta (polluted) and Hawkesbury (unpolluted) Rivers. The underlying genetics of the populations in these distinct waterways was examined at one mitochondrial (cytochrome c oxidase subunit I (COI)) and one nuclear (ribosomal internal transcribed spacer region 1 (ITS1)) locus. Seven unique haplotypes for COI were found amongst animals from the Parramatta River, while animals from the Hawkesbury River showed a complete absence of genetic variation at this locus. At ITS1 a total of two sequence variants were found amongst Parramatta River amphipods and three sequence variants among Hawkesbury River animals, with no common variants across the two river systems. To establish whether genetic differences were associated with organismal responses to toxicant exposure, two life-history trait variables (female head length as an estimator of amphipod size and female fecundity) were analyzed. Life-history trait analyses showed that females from the Hawkesbury River were significantly larger and more fecund. These data have critical implications for toxicity tests, the use of laboratory cultures for testing purposes, and environmental contamination in Sydney Harbor. © 2008 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/8780