Fluorophore labeling to monitor tRNA dynamics

Liu, C; Betteridge, T; Hou, Y

Fluorophore labeling to monitor tRNA dynamics

Liu, C Betteridge, T Hou, Y

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Publisher:: Academic Press
Publication Type:: Journal Article
Citation:: Methods in Enzymology, 2009, 46904 pp. 69 - 93
Issue Date:: 2009-01

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Field	Value	Language
dc.contributor.author	Liu, C	en_US
dc.contributor.author	Betteridge, T	en_US
dc.contributor.author	Hou, Y	en_US
dc.date.issued	2009-01	en_US
dc.identifier.citation	Methods in Enzymology, 2009, 46904 pp. 69 - 93	en_US
dc.identifier.issn	0076-6879	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8851
dc.description.abstract	Transfer RNA (tRNA) molecules mediate translation of the nucleic acid genetic code into the amino acid building blocks of proteins, thus ensuring the survivability of cells. The dynamic properties of tRNA molecules are crucial to their functions in both activity and specificity. This chapter summarizes two methods that have been recently developed or improved upon previous protocols to introduce fluorophores to site-specific positions in tRNA. One method enables incorporation of fluorophores carrying a primary amine (such as proflavin or rhodamine) to dihydrouridine (D) residues in the tRNA tertiary core, and a second method enables incorporation of pyrroloC and 2-aminopurine to positions 75 and 76, respectively, of the CCA sequence at the 30 end. These site-specific fluorophore labeling methods utilize tRNA transcripts as the substrates to provide the versatility with both wild-type and mutant sequences for examining their motions in space and time during the process of decoding genetic information.	en_US
dc.publisher	Academic Press	en_US
dc.relation.ispartof	Methods in Enzymology	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.title	Fluorophore labeling to monitor tRNA dynamics	en_US
dc.type	Journal Article
utslib.citation.volume	46904	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
dc.location.activity	ISI:000281557900007
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.consider-herdc	false	en_US
pubs.volume	46904	en_US

Abstract:

Transfer RNA (tRNA) molecules mediate translation of the nucleic acid genetic code into the amino acid building blocks of proteins, thus ensuring the survivability of cells. The dynamic properties of tRNA molecules are crucial to their functions in both activity and specificity. This chapter summarizes two methods that have been recently developed or improved upon previous protocols to introduce fluorophores to site-specific positions in tRNA. One method enables incorporation of fluorophores carrying a primary amine (such as proflavin or rhodamine) to dihydrouridine (D) residues in the tRNA tertiary core, and a second method enables incorporation of pyrroloC and 2-aminopurine to positions 75 and 76, respectively, of the CCA sequence at the 30 end. These site-specific fluorophore labeling methods utilize tRNA transcripts as the substrates to provide the versatility with both wild-type and mutant sequences for examining their motions in space and time during the process of decoding genetic information.

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http://hdl.handle.net/10453/8851