The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration

McGonigle, L; Mousley, A; Marks, NJ; Brennan, GP; Dalton, JP; Spithill, TW; Day, TA; Maule, AG

The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration

McGonigle, L Mousley, A Marks, NJ Brennan, GP Dalton, JP Spithill, TW Day, TA Maule, AG

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2008, 38 (2), pp. 149 - 155
Issue Date:: 2008-02-01

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Field	Value	Language
dc.contributor.author	McGonigle, L	en_US
dc.contributor.author	Mousley, A	en_US
dc.contributor.author	Marks, NJ	en_US
dc.contributor.author	Brennan, GP	en_US
dc.contributor.author	Dalton, JP	en_US
dc.contributor.author	Spithill, TW	en_US
dc.contributor.author	Day, TA	en_US
dc.contributor.author	Maule, AG	en_US
dc.date.available	2007-10-16	en_US
dc.date.issued	2008-02-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2008, 38 (2), pp. 149 - 155	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8909
dc.description.abstract	Probing protein function in parasitic flatworms is hampered by the difficulties associated with the development of transgenic approaches. Although RNA interference (RNAi) in schistosomes shows much promise, it has not been reported in other trematodes. Here, we show the successful silencing of the cysteine proteases cathepsin B and L in the infective stage of Fasciola hepatica newly excysted juveniles (NEJs). Silencing resulted in marked reductions in target transcript levels and significant diminution in the encoded proteins in the gut. RNAi of either enzyme in NEJs induced transient, abnormal locomotory phenotypes, and significantly reduced penetration of the rat intestinal wall. © 2007 Australian Society for Parasitology Inc.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/j.ijpara.2007.10.007	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Gastrointestinal Tract	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Rats, Wistar	en_US
dc.subject.mesh	Fasciola hepatica	en_US
dc.subject.mesh	Fascioliasis	en_US
dc.subject.mesh	Intestinal Diseases, Parasitic	en_US
dc.subject.mesh	Cathepsins	en_US
dc.subject.mesh	Cathepsin B	en_US
dc.subject.mesh	Cysteine Endopeptidases	en_US
dc.subject.mesh	Gene Silencing	en_US
dc.subject.mesh	RNA Interference	en_US
dc.subject.mesh	Locomotion	en_US
dc.subject.mesh	Cathepsin L	en_US
dc.subject.mesh	Genetic Therapy	en_US
dc.title	The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	38	en_US
utslib.for	0707 Veterinary Sciences	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
dc.location.activity	ISI:000253033900003	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	38	en_US

Abstract:

Probing protein function in parasitic flatworms is hampered by the difficulties associated with the development of transgenic approaches. Although RNA interference (RNAi) in schistosomes shows much promise, it has not been reported in other trematodes. Here, we show the successful silencing of the cysteine proteases cathepsin B and L in the infective stage of Fasciola hepatica newly excysted juveniles (NEJs). Silencing resulted in marked reductions in target transcript levels and significant diminution in the encoded proteins in the gut. RNAi of either enzyme in NEJs induced transient, abnormal locomotory phenotypes, and significantly reduced penetration of the rat intestinal wall. © 2007 Australian Society for Parasitology Inc.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/8909