Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging

Jones, AC; Milthorpe, B; Averdunk, H; Limaye, A; Senden, TJ; Sakellariou, A; Sheppard, AP; Sok, RM; Knackstedt, MA; Brandwood, A; Rohner, D; Hutmacher, DW

Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging

Jones, AC Milthorpe, B

Averdunk, H Limaye, A Senden, TJ Sakellariou, A Sheppard, AP Sok, RM Knackstedt, MA Brandwood, A Rohner, D Hutmacher, DW

Permalink

Publication Type:: Journal Article
Citation:: Biomaterials, 2004, 25 (20), pp. 4947 - 4954
Issue Date:: 2004-09-01

Closed Access

	Filename	Description	Size
	2008004380OK.pdf		1.52 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Jones, AC	en_US
dc.contributor.author	Milthorpe, B https://orcid.org/0000-0002-0867-9586	en_US
dc.contributor.author	Averdunk, H	en_US
dc.contributor.author	Limaye, A	en_US
dc.contributor.author	Senden, TJ	en_US
dc.contributor.author	Sakellariou, A	en_US
dc.contributor.author	Sheppard, AP	en_US
dc.contributor.author	Sok, RM	en_US
dc.contributor.author	Knackstedt, MA	en_US
dc.contributor.author	Brandwood, A	en_US
dc.contributor.author	Rohner, D	en_US
dc.contributor.author	Hutmacher, DW	en_US
dc.date.available	2004-01-20	en_US
dc.date.issued	2004-09-01	en_US
dc.identifier.citation	Biomaterials, 2004, 25 (20), pp. 4947 - 4954	en_US
dc.identifier.issn	0142-9612	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9258
dc.description.abstract	This paper illustrates the utility of micro-computed tomography (micro-CT) to study the process of tissue engineered bone growth. A micro-CT facility for imaging and visualising biomaterials in three dimensions (3D) is described. The facility is capable of acquiring 3D images made up of 20003 voxels on specimens up to 60mm in extent with resolutions down to 2μm. This allows the 3D structure of tissue engineered materials to be imaged across three orders of magnitude of detail. The capabilities of micro-CT are demonstrated by imaging the Haversian network within human femoral cortical bone (distal diaphysis) and bone ingrowth into a porous scaffold at varying resolutions. Phase identification combined with 3D visualisation enables one to observe the complex topology of the canalicular system of the cortical bone. Imaging of the tissue engineered bone at a scale of 1cm and resolutions of 10μm allows visualisation of the complex ingrowth of bone into the polymer scaffold. Further imaging at 2μm resolution allows observation of bone ultra-structure. These observations illustrate the benefits of tomography over traditional techniques for the characterisation of bone morphology and interconnectivity and performs a complimentary role to current histomorphometric techniques. © 2004 Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Biomaterials	en_US
dc.relation.isbasedon	10.1016/j.biomaterials.2004.01.047	en_US
dc.subject.classification	Biomedical Engineering	en_US
dc.subject.mesh	Bone and Bones	en_US
dc.subject.mesh	Femur	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Polymers	en_US
dc.subject.mesh	Bone Substitutes	en_US
dc.subject.mesh	Tomography, X-Ray Computed	en_US
dc.subject.mesh	Imaging, Three-Dimensional	en_US
dc.subject.mesh	Microradiography	en_US
dc.subject.mesh	Tissue Engineering	en_US
dc.subject.mesh	Bone Density	en_US
dc.subject.mesh	Models, Molecular	en_US
dc.subject.mesh	Image Processing, Computer-Assisted	en_US
dc.title	Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging	en_US
dc.type	Journal Article
utslib.citation.volume	20	en_US
utslib.citation.volume	25	en_US
utslib.for	0903 Biomedical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	20	en_US
pubs.publication-status	Published	en_US
pubs.volume	25	en_US

Abstract:

This paper illustrates the utility of micro-computed tomography (micro-CT) to study the process of tissue engineered bone growth. A micro-CT facility for imaging and visualising biomaterials in three dimensions (3D) is described. The facility is capable of acquiring 3D images made up of 20003 voxels on specimens up to 60mm in extent with resolutions down to 2μm. This allows the 3D structure of tissue engineered materials to be imaged across three orders of magnitude of detail. The capabilities of micro-CT are demonstrated by imaging the Haversian network within human femoral cortical bone (distal diaphysis) and bone ingrowth into a porous scaffold at varying resolutions. Phase identification combined with 3D visualisation enables one to observe the complex topology of the canalicular system of the cortical bone. Imaging of the tissue engineered bone at a scale of 1cm and resolutions of 10μm allows visualisation of the complex ingrowth of bone into the polymer scaffold. Further imaging at 2μm resolution allows observation of bone ultra-structure. These observations illustrate the benefits of tomography over traditional techniques for the characterisation of bone morphology and interconnectivity and performs a complimentary role to current histomorphometric techniques. © 2004 Elsevier Ltd. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/9258