The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum

Matthijs, M; Fabris, M; Obata, T; Foubert, I; Franco-Zorrilla, JM; Solano, R; Fernie, AR; Vyverman, W; Goossens, A

The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum

Matthijs, M Fabris, M

Obata, T Foubert, I Franco-Zorrilla, JM Solano, R

Fernie, AR Vyverman, W Goossens, A

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Publication Type:: Journal Article
Citation:: EMBO Journal, 2017, 36 (11), pp. 1559 - 1576
Issue Date:: 2017-06-01

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Field	Value	Language
dc.contributor.author	Matthijs, M	en_US
dc.contributor.author	Fabris, M https://orcid.org/0000-0003-2910-2089	en_US
dc.contributor.author	Obata, T	en_US
dc.contributor.author	Foubert, I	en_US
dc.contributor.author	Franco-Zorrilla, JM	en_US
dc.contributor.author	Solano, R https://orcid.org/0000-0001-5459-2417	en_US
dc.contributor.author	Fernie, AR	en_US
dc.contributor.author	Vyverman, W	en_US
dc.contributor.author	Goossens, A https://orcid.org/0000-0002-1599-551X	en_US
dc.date.available	2017-03-15	en_US
dc.date.issued	2017-06-01	en_US
dc.identifier.citation	EMBO Journal, 2017, 36 (11), pp. 1559 - 1576	en_US
dc.identifier.issn	0261-4189	en_US
dc.identifier.uri	http://hdl.handle.net/10453/93689
dc.description.abstract	© 2017 The Authors Diatoms are amongst the most important marine microalgae in terms of biomass, but little is known concerning the molecular mechanisms that regulate their versatile metabolism. Here, the pennate diatom Phaeodactylum tricornutum was studied at the metabolite and transcriptome level during nitrogen starvation and following imposition of three other stresses that impede growth. The coordinated upregulation of the tricarboxylic acid (TCA) cycle during the nitrogen stress response was the most striking observation. Through co-expression analysis and DNA binding assays, the transcription factor bZIP14 was identified as a regulator of the TCA cycle, also beyond the nitrogen starvation response, namely in diurnal regulation. Accordingly, metabolic and transcriptional shifts were observed upon overexpression of bZIP14 in transformed P. tricornutum cells. Our data indicate that the TCA cycle is a tightly regulated and important hub for carbon reallocation in the diatom cell during nutrient starvation and that bZIP14 is a conserved regulator of this cycle.	en_US
dc.relation.ispartof	EMBO Journal	en_US
dc.relation.isbasedon	10.15252/embj.201696392	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Diatoms	en_US
dc.subject.mesh	Carbon	en_US
dc.subject.mesh	Nitrogen	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Transcription, Genetic	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	Citric Acid Cycle	en_US
dc.subject.mesh	Circadian Rhythm	en_US
dc.subject.mesh	Stress, Physiological	en_US
dc.subject.mesh	Metabolome	en_US
dc.title	The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	36	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0802 Computation Theory and Mathematics	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	36	en_US

Abstract:

© 2017 The Authors Diatoms are amongst the most important marine microalgae in terms of biomass, but little is known concerning the molecular mechanisms that regulate their versatile metabolism. Here, the pennate diatom Phaeodactylum tricornutum was studied at the metabolite and transcriptome level during nitrogen starvation and following imposition of three other stresses that impede growth. The coordinated upregulation of the tricarboxylic acid (TCA) cycle during the nitrogen stress response was the most striking observation. Through co-expression analysis and DNA binding assays, the transcription factor bZIP14 was identified as a regulator of the TCA cycle, also beyond the nitrogen starvation response, namely in diurnal regulation. Accordingly, metabolic and transcriptional shifts were observed upon overexpression of bZIP14 in transformed P. tricornutum cells. Our data indicate that the TCA cycle is a tightly regulated and important hub for carbon reallocation in the diatom cell during nutrient starvation and that bZIP14 is a conserved regulator of this cycle.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/93689