Comparison of plasmids in strains of Zymomonas mobilis.

Stokes, HW; Dally, EL; Yablonsky, MD; Eveleigh, DE

Comparison of plasmids in strains of Zymomonas mobilis.

Stokes, HW Dally, EL Yablonsky, MD Eveleigh, DE

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Publication Type:: Journal Article
Citation:: Plasmid, 1983, 9 (2), pp. 138 - 146
Issue Date:: 1983-03

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Field	Value	Language
dc.contributor.author	Stokes, HW	en_US
dc.contributor.author	Dally, EL	en_US
dc.contributor.author	Yablonsky, MD	en_US
dc.contributor.author	Eveleigh, DE	en_US
dc.date.issued	1983-03	en_US
dc.identifier.citation	Plasmid, 1983, 9 (2), pp. 138 - 146	en_US
dc.identifier.issn	0147-619X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9425
dc.description.abstract	Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid (46 X 10(6) Da) and a smaller plasmid (16-21 X 10(6) Da) whose molecular weight was strain dependent. Two strains, Ag11 and ATCC 10988, had smaller plasmids of unique molecular weight. Homology existed between the plasmids in the four strains as shown by cross-reaction in DNA-DNA blot hybridizations. Only one plasmid appeared unique to the host from which it was isolated.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Plasmid	en_US
dc.relation.isbasedon	10.1016/0147-619x(83)90016-1	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Nucleic Acid Hybridization	en_US
dc.subject.mesh	Drug Resistance, Microbial	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Molecular Weight	en_US
dc.title	Comparison of plasmids in strains of Zymomonas mobilis.	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	9	en_US
utslib.location.activity	United States	en_US
utslib.for	100305 Industrial Microbiology (incl. Biofeedstocks)	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid (46 X 10(6) Da) and a smaller plasmid (16-21 X 10(6) Da) whose molecular weight was strain dependent. Two strains, Ag11 and ATCC 10988, had smaller plasmids of unique molecular weight. Homology existed between the plasmids in the four strains as shown by cross-reaction in DNA-DNA blot hybridizations. Only one plasmid appeared unique to the host from which it was isolated.

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http://hdl.handle.net/10453/9425