Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa

Semmler, ABT; Whitchurch, CB; Leech, AJ; Mattick, JS

Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa

Semmler, ABT Whitchurch, CB

Leech, AJ Mattick, JS

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Publication Type:: Journal Article
Citation:: Microbiology, 2000, 146 (6), pp. 1321 - 1332
Issue Date:: 2000-01-01

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Field	Value	Language
dc.contributor.author	Semmler, ABT	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Leech, AJ	en_US
dc.contributor.author	Mattick, JS	en_US
dc.date.issued	2000-01-01	en_US
dc.identifier.citation	Microbiology, 2000, 146 (6), pp. 1321 - 1332	en_US
dc.identifier.issn	1350-0872	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9507
dc.description.abstract	Transposon mutagenesis was used to identify a new locus required for twitching motility in Pseudomonas aeruginosa. Four Tn5-B21 mutants which lacked twitching motility and a fifth which exhibited impaired motility were found to map to the same KpnI restriction fragment at approximately 40 min on the P. aeruginosa genome. Cloning and sequencing studies showed that all five transposon insertions occurred within the same 2.8 kb ORF, which was termed fimV. The product of this gene has a putative peptidoglycan-binding domain, predicted transmembrane domains, a highly acidic C terminus and anomalous electrophoretic migration, indicating unusual primary or secondary structure. The P. aeruginosa genome also possesses a paralogue of fimV. Homologues of fimV were also found in the sequenced genomes of the other type-IV-fimbriated bacteria Neisseria gonorrhoeae, Neisseria meningitidis, Legionella pneumophila and Vibrio cholerae, but not in those of other bacteria which lack type IV fimbriae. A fimV homologue was also found in the genome sequence of Shewanella putrefaciens, along with many other homologues of type IV fimbrial genes, indicating that this bacterium is also likely to produce type IV fimbriae. Wild-type twitching motility was restored to fimV mutants by complementation in a dosage-dependent manner. Overexpression of fimV resulted in an unusual phenotype where the cells were massively elongated and migrated in large convoys at the periphery of the colony. It is suggested that FimV may be involved in remodelling of the peptidoglycan layer to enable assembly of the type IV fimbrial structure and machinery.	en_US
dc.relation.ispartof	Microbiology	en_US
dc.relation.isbasedon	10.1099/00221287-146-6-1321	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Restriction Mapping	en_US
dc.subject.mesh	Cloning, Molecular	en_US
dc.subject.mesh	Genetic Complementation Test	en_US
dc.subject.mesh	Mutagenesis, Insertional	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Movement	en_US
dc.subject.mesh	Phenotype	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	146	en_US
utslib.for	1108 Medical Microbiology	en_US
dc.location.activity	ISI:000087598700011	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	146	en_US

Abstract:

Transposon mutagenesis was used to identify a new locus required for twitching motility in Pseudomonas aeruginosa. Four Tn5-B21 mutants which lacked twitching motility and a fifth which exhibited impaired motility were found to map to the same KpnI restriction fragment at approximately 40 min on the P. aeruginosa genome. Cloning and sequencing studies showed that all five transposon insertions occurred within the same 2.8 kb ORF, which was termed fimV. The product of this gene has a putative peptidoglycan-binding domain, predicted transmembrane domains, a highly acidic C terminus and anomalous electrophoretic migration, indicating unusual primary or secondary structure. The P. aeruginosa genome also possesses a paralogue of fimV. Homologues of fimV were also found in the sequenced genomes of the other type-IV-fimbriated bacteria Neisseria gonorrhoeae, Neisseria meningitidis, Legionella pneumophila and Vibrio cholerae, but not in those of other bacteria which lack type IV fimbriae. A fimV homologue was also found in the genome sequence of Shewanella putrefaciens, along with many other homologues of type IV fimbrial genes, indicating that this bacterium is also likely to produce type IV fimbriae. Wild-type twitching motility was restored to fimV mutants by complementation in a dosage-dependent manner. Overexpression of fimV resulted in an unusual phenotype where the cells were massively elongated and migrated in large convoys at the periphery of the colony. It is suggested that FimV may be involved in remodelling of the peptidoglycan layer to enable assembly of the type IV fimbrial structure and machinery.

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http://hdl.handle.net/10453/9507