Intersexual variations in Northern (Missulena pruinosa) and Eastern (M. bradleyi) mouse spider venom

Herzig, V; Khalife, AA; Chong, Y; Isbister, GK; Currie, BJ; Churchill, TB; Horner, S; Escoubas, P; Nicholson, GM; Hodgson, WC

Intersexual variations in Northern (Missulena pruinosa) and Eastern (M. bradleyi) mouse spider venom

Herzig, V Khalife, AA Chong, Y Isbister, GK Currie, BJ Churchill, TB Horner, S Escoubas, P Nicholson, GM

Hodgson, WC

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Publication Type:: Journal Article
Citation:: Toxicon, 2008, 51 (7), pp. 1167 - 1177
Issue Date:: 2008-06-01

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Field	Value	Language
dc.contributor.author	Herzig, V	en_US
dc.contributor.author	Khalife, AA	en_US
dc.contributor.author	Chong, Y	en_US
dc.contributor.author	Isbister, GK	en_US
dc.contributor.author	Currie, BJ	en_US
dc.contributor.author	Churchill, TB	en_US
dc.contributor.author	Horner, S	en_US
dc.contributor.author	Escoubas, P	en_US
dc.contributor.author	Nicholson, GM https://orcid.org/0000-0002-4277-4296	en_US
dc.contributor.author	Hodgson, WC	en_US
dc.date.available	2008-02-01	en_US
dc.date.issued	2008-06-01	en_US
dc.identifier.citation	Toxicon, 2008, 51 (7), pp. 1167 - 1177	en_US
dc.identifier.issn	0041-0101	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9511
dc.description.abstract	Venoms of both sexes of Australian Northern (Missulena pruinosa) and Eastern (Missulena bradleyi) mouse spiders were studied in order to determine intersexual variations in venom yield, composition and bioactivity. Females of both species yielded more venom than males. High-performance liquid chromatography (HPLC) and mass spectrometry data further indicate a substantial degree of intersexual variation in the venom composition of both species. In a cricket (Acheta domestica) acute toxicity assay, only small intersexual differences were observed, but M. bradleyi venom was found to be considerably more potent than M. pruinosa venom. In the chick biventer cervicis nerve-muscle preparation, male but not female M. bradleyi venom induced large and sustained muscle contractions with fasciculation and decreased twitch height that could be reversed by CSL funnel-web spider antivenom. In contrast, venoms of both sexes of M. pruinosa did not induce significant effects in the chick biventer cervicis nerve-muscle preparation. We therefore conclude that female M. bradleyi venom and venoms from male and female M. pruinosa appear to contain few, if any, orthologs of δ-missulenatoxin-Mb1a, the toxin responsible for the effects of male M. bradleyi venom in vertebrates. These findings are consistent with clinical reports that mouse spiders, particularly species other than male M. bradleyi, do not appear to be a major medical problem in humans. © 2008 Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Toxicon	en_US
dc.relation.isbasedon	10.1016/j.toxicon.2008.02.001	en_US
dc.subject.classification	Toxicology	en_US
dc.subject.mesh	Muscle, Skeletal	en_US
dc.subject.mesh	Peripheral Nerves	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Chickens	en_US
dc.subject.mesh	Spiders	en_US
dc.subject.mesh	Gryllidae	en_US
dc.subject.mesh	Spider Venoms	en_US
dc.subject.mesh	Neurotoxins	en_US
dc.subject.mesh	Antivenins	en_US
dc.subject.mesh	Chromatography, High Pressure Liquid	en_US
dc.subject.mesh	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization	en_US
dc.subject.mesh	Toxicity Tests	en_US
dc.subject.mesh	Sex Factors	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Muscle Contraction	en_US
dc.subject.mesh	Dose-Response Relationship, Drug	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.title	Intersexual variations in Northern (Missulena pruinosa) and Eastern (M. bradleyi) mouse spider venom	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	51	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
dc.location.activity	ISI:000256999600005	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	51	en_US

Abstract:

Venoms of both sexes of Australian Northern (Missulena pruinosa) and Eastern (Missulena bradleyi) mouse spiders were studied in order to determine intersexual variations in venom yield, composition and bioactivity. Females of both species yielded more venom than males. High-performance liquid chromatography (HPLC) and mass spectrometry data further indicate a substantial degree of intersexual variation in the venom composition of both species. In a cricket (Acheta domestica) acute toxicity assay, only small intersexual differences were observed, but M. bradleyi venom was found to be considerably more potent than M. pruinosa venom. In the chick biventer cervicis nerve-muscle preparation, male but not female M. bradleyi venom induced large and sustained muscle contractions with fasciculation and decreased twitch height that could be reversed by CSL funnel-web spider antivenom. In contrast, venoms of both sexes of M. pruinosa did not induce significant effects in the chick biventer cervicis nerve-muscle preparation. We therefore conclude that female M. bradleyi venom and venoms from male and female M. pruinosa appear to contain few, if any, orthologs of δ-missulenatoxin-Mb1a, the toxin responsible for the effects of male M. bradleyi venom in vertebrates. These findings are consistent with clinical reports that mouse spiders, particularly species other than male M. bradleyi, do not appear to be a major medical problem in humans. © 2008 Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/9511