Development and application of a rapid amphipod reproduction test for sediment-quality assessment

Mann, RM; Hyne, RV; Spadaro, DA; Simpson, SL

Development and application of a rapid amphipod reproduction test for sediment-quality assessment

Mann, RM

Hyne, RV Spadaro, DA Simpson, SL

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Publication Type:: Journal Article
Citation:: Environmental Toxicology and Chemistry, 2009, 28 (6), pp. 1244 - 1254
Issue Date:: 2009-06-01

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Field	Value	Language
dc.contributor.author	Mann, RM https://orcid.org/0000-0001-7696-0751	en_US
dc.contributor.author	Hyne, RV	en_US
dc.contributor.author	Spadaro, DA	en_US
dc.contributor.author	Simpson, SL	en_US
dc.date.available	2008-12-09	en_US
dc.date.issued	2009-06-01	en_US
dc.identifier.citation	Environmental Toxicology and Chemistry, 2009, 28 (6), pp. 1244 - 1254	en_US
dc.identifier.issn	0730-7268	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9612
dc.description.abstract	Melita plumulosa is an epibenthic, detritivorous amphipod native to eastern Australia that has been adopted as a test organism for toxicity evaluations of contaminated estuarine sediments. In the present study, a 13-d amphipod reproduction test was developed that encompasses gametogenesis, fertilization, and embryo development before hatching. The primary endpoints for the test are fecundity (measured as the number of embryos per individual surviving female) and a fecundity index (fecundity multiplied by the stage of embryo development). This new test has been employed to scrutinize the sediments from a metal-contaminated coastal lagoon. Lake Macquarie (NSW, Australia) is a large, saltwater lagoon that has received metal pollution over many decades, leading to a concentration gradient of trace metals, including Pb, Zn, Cd, and Cu, in the sediments. Within one of the northern bays (Warners Bay), the concentrations of these metals either border on or exceed sediment quality guideline values prescribed by Australian and New Zealand Guidelines for Fresh and Marine Water Quality. In trials with the 13-d amphipod reproduction test, Warners Bay sediments significantly reduced fecundity in the test species. Subsequent tests with clean sediments spiked singly with Pb, Zn, or Cu indicated that no single metal was responsible for the observed toxicity in the field sediments. However, sediments spiked with various combinations of Pb, Zn, Cd, and Cu indicated that Zn in combination with one or more of the other metals was responsible for the reproductive toxicity observed in Warners Bay sediments. In all these tests, measured metal concentrations in overlying water and pore water were low, thus confirming that the observed effects on reproduction could be attributed to dietary exposure to metals. © 2009 SETAC.	en_US
dc.relation.ispartof	Environmental Toxicology and Chemistry	en_US
dc.relation.isbasedon	10.1897/08-346.1	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Amphipoda	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Reproduction	en_US
dc.subject.mesh	Geologic Sediments	en_US
dc.title	Development and application of a rapid amphipod reproduction test for sediment-quality assessment	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	28	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0602 Ecology	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	28	en_US

Abstract:

Melita plumulosa is an epibenthic, detritivorous amphipod native to eastern Australia that has been adopted as a test organism for toxicity evaluations of contaminated estuarine sediments. In the present study, a 13-d amphipod reproduction test was developed that encompasses gametogenesis, fertilization, and embryo development before hatching. The primary endpoints for the test are fecundity (measured as the number of embryos per individual surviving female) and a fecundity index (fecundity multiplied by the stage of embryo development). This new test has been employed to scrutinize the sediments from a metal-contaminated coastal lagoon. Lake Macquarie (NSW, Australia) is a large, saltwater lagoon that has received metal pollution over many decades, leading to a concentration gradient of trace metals, including Pb, Zn, Cd, and Cu, in the sediments. Within one of the northern bays (Warners Bay), the concentrations of these metals either border on or exceed sediment quality guideline values prescribed by Australian and New Zealand Guidelines for Fresh and Marine Water Quality. In trials with the 13-d amphipod reproduction test, Warners Bay sediments significantly reduced fecundity in the test species. Subsequent tests with clean sediments spiked singly with Pb, Zn, or Cu indicated that no single metal was responsible for the observed toxicity in the field sediments. However, sediments spiked with various combinations of Pb, Zn, Cd, and Cu indicated that Zn in combination with one or more of the other metals was responsible for the reproductive toxicity observed in Warners Bay sediments. In all these tests, measured metal concentrations in overlying water and pore water were low, thus confirming that the observed effects on reproduction could be attributed to dietary exposure to metals. © 2009 SETAC.

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http://hdl.handle.net/10453/9612