Destruction and control of Toxoplasma gondii tachyzoites using gold nanosphere/antibody conjugates

Pissuwan, D; Valenzuela, SM; Miller, CM; Killingsworth, MC; Cortie, MB

Destruction and control of Toxoplasma gondii tachyzoites using gold nanosphere/antibody conjugates

Pissuwan, D Valenzuela, SM

Miller, CM

Killingsworth, MC Cortie, MB

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Publication Type:: Journal Article
Citation:: Small, 2009, 5 (9), pp. 1030 - 1034
Issue Date:: 2009-05-04

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Field	Value	Language
dc.contributor.author	Pissuwan, D	en_US
dc.contributor.author	Valenzuela, SM https://orcid.org/0000-0001-5934-6047	en_US
dc.contributor.author	Miller, CM https://orcid.org/0000-0001-6071-5102	en_US
dc.contributor.author	Killingsworth, MC	en_US
dc.contributor.author	Cortie, MB https://orcid.org/0000-0003-3202-6398	en_US
dc.date.issued	2009-05-04	en_US
dc.identifier.citation	Small, 2009, 5 (9), pp. 1030 - 1034	en_US
dc.identifier.issn	1613-6810	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9631
dc.description.abstract	The targeting and destruction of Toxoplasma gondii (T.gondii) tachyzoites was studied to be achieved with simple antibody-functionalized gold nanospheres. The nanospheres of approximately 20-nm diameter were conjugated to an antibody specified to T.gondii to produce a gold/antibody conjugate. Microscopic imaging and optical properties indicate the presence of 3000 to 3500 gold nanospheres per tachyzoite. There is no significant photothermal destruction of tachyzoites observed in the absence of Au/anti-T.g. and in this case the number of dead tachyzoites did not increase when the laser dose is increased from 900 to 2100J cm-3. The results also show that an irradiation of 1800J cm -3 caused a cell death rate of 13.5%∓3.6%. The percentage of CHO-K1 cells infected by tachyzoites is inhibited when they are incubated with anti-T.G. alone.	en_US
dc.relation.ispartof	Small	en_US
dc.relation.isbasedon	10.1002/smll.200801018	en_US
dc.subject.classification	Nanoscience & Nanotechnology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Toxoplasma	en_US
dc.subject.mesh	Gold	en_US
dc.subject.mesh	Antibodies	en_US
dc.subject.mesh	Hyperthermia, Induced	en_US
dc.subject.mesh	Light	en_US
dc.subject.mesh	Nanomedicine	en_US
dc.subject.mesh	Nanospheres	en_US
dc.title	Destruction and control of Toxoplasma gondii tachyzoites using gold nanosphere/antibody conjugates	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	5	en_US
utslib.for	0912 Materials Engineering	en_US
dc.location.activity	ISI:000266184500006	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

The targeting and destruction of Toxoplasma gondii (T.gondii) tachyzoites was studied to be achieved with simple antibody-functionalized gold nanospheres. The nanospheres of approximately 20-nm diameter were conjugated to an antibody specified to T.gondii to produce a gold/antibody conjugate. Microscopic imaging and optical properties indicate the presence of 3000 to 3500 gold nanospheres per tachyzoite. There is no significant photothermal destruction of tachyzoites observed in the absence of Au/anti-T.g. and in this case the number of dead tachyzoites did not increase when the laser dose is increased from 900 to 2100J cm-3. The results also show that an irradiation of 1800J cm -3 caused a cell death rate of 13.5%∓3.6%. The percentage of CHO-K1 cells infected by tachyzoites is inhibited when they are incubated with anti-T.G. alone.

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http://hdl.handle.net/10453/9631