Clonal complexes of Campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaA gene

Djordjevic, SP; Unicomb, LE; Adamson, PJ; Mickan, L; Rios, R; Adamson, P; Cheung, K; Combs, B; Dalton, C; Doyle, R; Ferguson, J; Gilbert, L; Givney, R; Gordon, D; Gregory, J; Hogg, G; Inglis, T; Jelfs, P; Kirk, M; Lalor, K; Lanser, J; O'Reilly, L; Sarna, M; Sharma, H; Smith, H; Valcanis, M

Clonal complexes of Campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaA gene

Unicomb, LE Adamson, PJ Mickan, L Rios, R Adamson, P Cheung, K Combs, B Dalton, C Doyle, R Ferguson, J Gilbert, L Givney, R Gordon, D Gregory, J Hogg, G Inglis, T Jelfs, P Kirk, M Lalor, K Lanser, J O'Reilly, L Sarna, M Sharma, H Smith, H Valcanis, M

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Publication Type:: Journal Article
Citation:: Journal of Clinical Microbiology, 2007, 45 (1), pp. 102 - 108
Issue Date:: 2007-01-01

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Field	Value	Language
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Unicomb, LE	en_US
dc.contributor.author	Adamson, PJ	en_US
dc.contributor.author	Mickan, L	en_US
dc.contributor.author	Rios, R	en_US
dc.contributor.author	Adamson, P	en_US
dc.contributor.author	Cheung, K	en_US
dc.contributor.author	Combs, B	en_US
dc.contributor.author	Dalton, C	en_US
dc.contributor.author	Doyle, R	en_US
dc.contributor.author	Ferguson, J	en_US
dc.contributor.author	Gilbert, L	en_US
dc.contributor.author	Givney, R	en_US
dc.contributor.author	Gordon, D	en_US
dc.contributor.author	Gregory, J	en_US
dc.contributor.author	Hogg, G	en_US
dc.contributor.author	Inglis, T	en_US
dc.contributor.author	Jelfs, P	en_US
dc.contributor.author	Kirk, M	en_US
dc.contributor.author	Lalor, K	en_US
dc.contributor.author	Lanser, J	en_US
dc.contributor.author	O'Reilly, L	en_US
dc.contributor.author	Sarna, M	en_US
dc.contributor.author	Sharma, H	en_US
dc.contributor.author	Smith, H	en_US
dc.contributor.author	Valcanis, M	en_US
dc.date.issued	2007-01-01	en_US
dc.identifier.citation	Journal of Clinical Microbiology, 2007, 45 (1), pp. 102 - 108	en_US
dc.identifier.issn	0095-1137	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9728
dc.description.abstract	Multilocus sequence typing (MLST) has provided important new insights into the population structure of Campylobacter jejuni and is rapidly becoming the gold standard for typing this species. However, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. Restriction fragment length polymorphism analysis of the flaA gene (RELP-flaA) and sequencing of the variable region in the fla locus (SVR-fla) were compared to MLST to determine if a low cost alternative could be found that reliably predicts clonal lineage (as determined by MLST). An isolate of C. jejuni from each of 153 patients from New South Wales, Australia, collected sequentially over a period of 30 months from 1999 to 2001 and comprising 40 sequence types (ST) from 15 clonal complexes (CC) was examined. Of 15 CC, 12 were represented by more than one isolate and a predominant RFLP-flaA type was found for 10 (83%). Of these, seven (70%) correctly predicted the predominant MLST CC with a probability of >0.8. Of 40 STs detected, 19 were reported for the first time, 9 of which were represented by more than one isolate. Eight of these were represented by a single RFLP-flaA type. Only two of eight major SVR-fla types were able to predict CC with a probability of >0.8, indicating that flaA-RFLP is a more reliable predictor of CC than SVR-fla and thus offers an alternative to MLST for use in routine surveillance. Copyright © 2007, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Journal of Clinical Microbiology	en_US
dc.relation.isbasedon	10.1128/JCM.01012-06	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Campylobacter jejuni	en_US
dc.subject.mesh	Campylobacter Infections	en_US
dc.subject.mesh	Flagellin	en_US
dc.subject.mesh	Bacterial Typing Techniques	en_US
dc.subject.mesh	Population Surveillance	en_US
dc.subject.mesh	Predictive Value of Tests	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Polymorphism, Restriction Fragment Length	en_US
dc.title	Clonal complexes of Campylobacter jejuni identified by multilocus sequence typing are reliably predicted by restriction fragment length polymorphism analyses of the flaA gene	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	45	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	45	en_US

Abstract:

Multilocus sequence typing (MLST) has provided important new insights into the population structure of Campylobacter jejuni and is rapidly becoming the gold standard for typing this species. However, the methodology is comparatively costly and slow to perform for the routine surveillance testing of large numbers of isolates required by public health laboratories. Restriction fragment length polymorphism analysis of the flaA gene (RELP-flaA) and sequencing of the variable region in the fla locus (SVR-fla) were compared to MLST to determine if a low cost alternative could be found that reliably predicts clonal lineage (as determined by MLST). An isolate of C. jejuni from each of 153 patients from New South Wales, Australia, collected sequentially over a period of 30 months from 1999 to 2001 and comprising 40 sequence types (ST) from 15 clonal complexes (CC) was examined. Of 15 CC, 12 were represented by more than one isolate and a predominant RFLP-flaA type was found for 10 (83%). Of these, seven (70%) correctly predicted the predominant MLST CC with a probability of >0.8. Of 40 STs detected, 19 were reported for the first time, 9 of which were represented by more than one isolate. Eight of these were represented by a single RFLP-flaA type. Only two of eight major SVR-fla types were able to predict CC with a probability of >0.8, indicating that flaA-RFLP is a more reliable predictor of CC than SVR-fla and thus offers an alternative to MLST for use in routine surveillance. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/9728