Gold nanoparticles improve metabolic profile of mice fed a high-fat diet

Chen, H; Ng, JPM; Tan, Y; McGrath, K; Bishop, DP; Oliver, B; Chan, YL; Cortie, MB; Milthorpe, BK; Valenzuela, SM

Gold nanoparticles improve metabolic profile of mice fed a high-fat diet

Chen, H

Ng, JPM Tan, Y

McGrath, K

Bishop, DP

Oliver, B

Chan, YL

Cortie, MB

Milthorpe, BK

Valenzuela, SM

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Publication Type:: Journal Article
Citation:: Journal of Nanobiotechnology, 2018, 16 (1)
Issue Date:: 2018-02-06

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Field	Value	Language
dc.contributor.author	Chen, H https://orcid.org/0000-0001-6883-3752	en_US
dc.contributor.author	Ng, JPM	en_US
dc.contributor.author	Tan, Y https://orcid.org/0000-0001-9221-330X	en_US
dc.contributor.author	McGrath, K https://orcid.org/0000-0002-6244-3929	en_US
dc.contributor.author	Bishop, DP https://orcid.org/0000-0002-6533-4410	en_US
dc.contributor.author	Oliver, B https://orcid.org/0000-0002-7122-9262	en_US
dc.contributor.author	Chan, YL https://orcid.org/0000-0002-9605-4200	en_US
dc.contributor.author	Cortie, MB https://orcid.org/0000-0003-3202-6398	en_US
dc.contributor.author	Milthorpe, BK https://orcid.org/0000-0002-0867-9586	en_US
dc.contributor.author	Valenzuela, SM https://orcid.org/0000-0001-5934-6047	en_US
dc.date.available	2018-01-27	en_US
dc.date.issued	2018-02-06	en_US
dc.identifier.citation	Journal of Nanobiotechnology, 2018, 16 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/122109
dc.description.abstract	© 2018 The Author(s). Background: Obesity is a high risk for multiple metabolic disorders due to excessive influx of energy, glucose and lipid, often from a western based diet. Low-grade inflammation plays a key role in the progression of such metabolic disorders. The anti-inflammatory property of gold compounds has been used in treating rheumatoid arthritis in the clinic. Previously we found that pure gold nanoparticles (AuNPs, 21 nm) also possess anti-inflammatory effects on the retroperitoneal fat tissue following intraperitoneal injection, by downregulating tumor necrosis factor (TNF) α. However, whether such an effect can change the risk of metabolic disorders in the obese has not been well studied. The study employed C57BL/6 mice fed a pellet high fat diet (HFD, 43% as fat) that were treated daily with AuNPs [low (HFD-LAu) or high (HFD-HAu) dose] via intraperitoneal injection for 9 weeks. In the in vitro study, RAW264.7 macrophages and 3T3-L1 adipocytes were cultured with low and high concentrations of AuNPs alone or together. Results: The HFD-fed mice showed a significant increase in fat mass, glucose intolerance, dyslipidemia, and liver steatosis. The HFD-LAu group showed an 8% reduction in body weight, ameliorated hyperlipidemia, and normal glucose tolerance; while the HFD-HAu group had a 5% reduction in body weight with significant improvement in their glucose intolerance and hyperlipidemia. The underlying mechanism may be attributed to a reduction in adipose and hepatic local proinflammatory cytokine production, e.g. TNFα. In vitro studies of co-cultured murine RAW264.7 macrophage and 3T3-L1 adipocytes supported this proposed mechanism. Conclusion: AuNPs demonstrate a promising profile for potential management of obesity related glucose and lipid disorders and are useful as a research tool for the study of biological mechanisms.	en_US
dc.relation.ispartof	Journal of Nanobiotechnology	en_US
dc.relation.isbasedon	10.1186/s12951-018-0338-1	en_US
dc.subject.classification	Nanoscience & Nanotechnology	en_US
dc.subject.mesh	Liver	en_US
dc.subject.mesh	3T3-L1 Cells	en_US
dc.subject.mesh	Adipocytes	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred C57BL	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Inflammation	en_US
dc.subject.mesh	Gold	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Glucose Tolerance Test	en_US
dc.subject.mesh	Coculture Techniques	en_US
dc.subject.mesh	Tissue Distribution	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Metal Nanoparticles	en_US
dc.subject.mesh	Metabolomics	en_US
dc.subject.mesh	Metabolome	en_US
dc.subject.mesh	Diet, High-Fat	en_US
dc.subject.mesh	Biomarkers	en_US
dc.title	Gold nanoparticles improve metabolic profile of mice fed a high-fat diet	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	16	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	1004 Medical Biotechnology	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	16	en_US

Abstract:

© 2018 The Author(s). Background: Obesity is a high risk for multiple metabolic disorders due to excessive influx of energy, glucose and lipid, often from a western based diet. Low-grade inflammation plays a key role in the progression of such metabolic disorders. The anti-inflammatory property of gold compounds has been used in treating rheumatoid arthritis in the clinic. Previously we found that pure gold nanoparticles (AuNPs, 21 nm) also possess anti-inflammatory effects on the retroperitoneal fat tissue following intraperitoneal injection, by downregulating tumor necrosis factor (TNF) α. However, whether such an effect can change the risk of metabolic disorders in the obese has not been well studied. The study employed C57BL/6 mice fed a pellet high fat diet (HFD, 43% as fat) that were treated daily with AuNPs [low (HFD-LAu) or high (HFD-HAu) dose] via intraperitoneal injection for 9 weeks. In the in vitro study, RAW264.7 macrophages and 3T3-L1 adipocytes were cultured with low and high concentrations of AuNPs alone or together. Results: The HFD-fed mice showed a significant increase in fat mass, glucose intolerance, dyslipidemia, and liver steatosis. The HFD-LAu group showed an 8% reduction in body weight, ameliorated hyperlipidemia, and normal glucose tolerance; while the HFD-HAu group had a 5% reduction in body weight with significant improvement in their glucose intolerance and hyperlipidemia. The underlying mechanism may be attributed to a reduction in adipose and hepatic local proinflammatory cytokine production, e.g. TNFα. In vitro studies of co-cultured murine RAW264.7 macrophage and 3T3-L1 adipocytes supported this proposed mechanism. Conclusion: AuNPs demonstrate a promising profile for potential management of obesity related glucose and lipid disorders and are useful as a research tool for the study of biological mechanisms.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/122109