Nasal gene expression differentiates COPD from controls and overlaps bronchial gene expression

Boudewijn, IM; Faiz, A; Steiling, K; van der Wiel, E; Telenga, ED; Hoonhorst, SJM; ten Hacken, NHT; Brandsma, CA; Kerstjens, HAM; Timens, W; Heijink, IH; Jonker, MR; de Bruin, HG; Sebastiaan Vroegop, J; Pasma, HR; Boersma, WG; Wielders, P; van den Elshout, F; Mansour, K; Spira, A; Lenburg, ME; Guryev, V; Postma, DS; van den Berge, M

Nasal gene expression differentiates COPD from controls and overlaps bronchial gene expression

Boudewijn, IM Faiz, A

Steiling, K van der Wiel, E Telenga, ED Hoonhorst, SJM ten Hacken, NHT Brandsma, CA Kerstjens, HAM Timens, W Heijink, IH Jonker, MR de Bruin, HG Sebastiaan Vroegop, J Pasma, HR Boersma, WG Wielders, P van den Elshout, F Mansour, K Spira, A Lenburg, ME Guryev, V Postma, DS van den Berge, M

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Publication Type:: Journal Article
Citation:: Respiratory Research, 2017, 18 (1)
Issue Date:: 2017-12-21

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Field	Value	Language
dc.contributor.author	Boudewijn, IM	en_US
dc.contributor.author	Faiz, A https://orcid.org/0000-0003-1740-3538	en_US
dc.contributor.author	Steiling, K	en_US
dc.contributor.author	van der Wiel, E	en_US
dc.contributor.author	Telenga, ED	en_US
dc.contributor.author	Hoonhorst, SJM	en_US
dc.contributor.author	ten Hacken, NHT	en_US
dc.contributor.author	Brandsma, CA	en_US
dc.contributor.author	Kerstjens, HAM	en_US
dc.contributor.author	Timens, W	en_US
dc.contributor.author	Heijink, IH	en_US
dc.contributor.author	Jonker, MR	en_US
dc.contributor.author	de Bruin, HG	en_US
dc.contributor.author	Sebastiaan Vroegop, J	en_US
dc.contributor.author	Pasma, HR	en_US
dc.contributor.author	Boersma, WG	en_US
dc.contributor.author	Wielders, P	en_US
dc.contributor.author	van den Elshout, F	en_US
dc.contributor.author	Mansour, K	en_US
dc.contributor.author	Spira, A	en_US
dc.contributor.author	Lenburg, ME	en_US
dc.contributor.author	Guryev, V	en_US
dc.contributor.author	Postma, DS	en_US
dc.contributor.author	van den Berge, M	en_US
dc.date.available	2017-12-11	en_US
dc.date.issued	2017-12-21	en_US
dc.identifier.citation	Respiratory Research, 2017, 18 (1)	en_US
dc.identifier.issn	1465-9921	en_US
dc.identifier.uri	http://hdl.handle.net/10453/128484
dc.description.abstract	© 2017 The Author(s). Background: Nasal gene expression profiling is a promising method to characterize COPD non-invasively. We aimed to identify a nasal gene expression profile to distinguish COPD patients from healthy controls. We investigated whether this COPD-associated gene expression profile in nasal epithelium is comparable with the profile observed in bronchial epithelium. Methods: Genome wide gene expression analysis was performed on nasal epithelial brushes of 31 severe COPD patients and 22 controls, all current smokers, using Affymetrix Human Gene 1.0 ST Arrays. We repeated the gene expression analysis on bronchial epithelial brushes in 2 independent cohorts of mild-to-moderate COPD patients and controls. Results: In nasal epithelium, 135 genes were significantly differentially expressed between severe COPD patients and controls, 21 being up- and 114 downregulated in COPD (false discovery rate < 0.01). Gene Set Enrichment Analysis (GSEA) showed significant concordant enrichment of COPD-associated nasal and bronchial gene expression in both independent cohorts (FDRGSEA < 0.001). Conclusion: We identified a nasal gene expression profile that differentiates severe COPD patients from controls. Of interest, part of the nasal gene expression changes in COPD mimics differentially expressed genes in the bronchus. These findings indicate that nasal gene expression profiling is potentially useful as a non-invasive biomarker in COPD. Trial registration:ClinicalTrials.govregistration number NCT01351792(registration date May 10, 2011), ClinicalTrials.govregistration number NCT00848406(registration date February 19, 2009), ClinicalTrials.govregistration number NCT00807469(registration date December 11, 2008).	en_US
dc.relation.ispartof	Respiratory Research	en_US
dc.relation.isbasedon	10.1186/s12931-017-0696-5	en_US
dc.subject.classification	Respiratory System	en_US
dc.subject.mesh	Bronchi	en_US
dc.subject.mesh	Nasal Mucosa	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Pulmonary Disease, Chronic Obstructive	en_US
dc.subject.mesh	Cohort Studies	en_US
dc.subject.mesh	Gene Expression	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Aged	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.title	Nasal gene expression differentiates COPD from controls and overlaps bronchial gene expression	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	18	en_US
utslib.for	1102 Cardiorespiratory Medicine and Haematology	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	18	en_US

Abstract:

© 2017 The Author(s). Background: Nasal gene expression profiling is a promising method to characterize COPD non-invasively. We aimed to identify a nasal gene expression profile to distinguish COPD patients from healthy controls. We investigated whether this COPD-associated gene expression profile in nasal epithelium is comparable with the profile observed in bronchial epithelium. Methods: Genome wide gene expression analysis was performed on nasal epithelial brushes of 31 severe COPD patients and 22 controls, all current smokers, using Affymetrix Human Gene 1.0 ST Arrays. We repeated the gene expression analysis on bronchial epithelial brushes in 2 independent cohorts of mild-to-moderate COPD patients and controls. Results: In nasal epithelium, 135 genes were significantly differentially expressed between severe COPD patients and controls, 21 being up- and 114 downregulated in COPD (false discovery rate < 0.01). Gene Set Enrichment Analysis (GSEA) showed significant concordant enrichment of COPD-associated nasal and bronchial gene expression in both independent cohorts (FDRGSEA < 0.001). Conclusion: We identified a nasal gene expression profile that differentiates severe COPD patients from controls. Of interest, part of the nasal gene expression changes in COPD mimics differentially expressed genes in the bronchus. These findings indicate that nasal gene expression profiling is potentially useful as a non-invasive biomarker in COPD. Trial registration:ClinicalTrials.govregistration number NCT01351792(registration date May 10, 2011), ClinicalTrials.govregistration number NCT00848406(registration date February 19, 2009), ClinicalTrials.govregistration number NCT00807469(registration date December 11, 2008).

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http://hdl.handle.net/10453/128484