MicroRNA profiling in lung tissue and bronchoalveolar lavage of cigarette smoke-exposed mice and in COPD patients: A translational approach

Conickx, G; Avila Cobos, F; Van Den Berge, M; Faiz, A; Timens, W; Hiemstra, PS; Joos, GF; Brusselle, GG; Mestdagh, P; Bracke, KR

MicroRNA profiling in lung tissue and bronchoalveolar lavage of cigarette smoke-exposed mice and in COPD patients: A translational approach

Conickx, G Avila Cobos, F Van Den Berge, M Faiz, A

Timens, W Hiemstra, PS Joos, GF Brusselle, GG Mestdagh, P Bracke, KR

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2017, 7 (1)
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Conickx, G	en_US
dc.contributor.author	Avila Cobos, F	en_US
dc.contributor.author	Van Den Berge, M	en_US
dc.contributor.author	Faiz, A https://orcid.org/0000-0003-1740-3538	en_US
dc.contributor.author	Timens, W	en_US
dc.contributor.author	Hiemstra, PS	en_US
dc.contributor.author	Joos, GF	en_US
dc.contributor.author	Brusselle, GG	en_US
dc.contributor.author	Mestdagh, P	en_US
dc.contributor.author	Bracke, KR	en_US
dc.date.available	2017-09-20	en_US
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	Scientific Reports, 2017, 7 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/129314
dc.description.abstract	© 2017 The Author(s). Chronic obstructive pulmonary disease (COPD) is characterized by a progressive airflow limitation and is associated with a chronic inflammatory response in both airways and lungs. microRNAs (miRNAs) are often highly conserved between species and have an intricate role within homeostatic conditions and immune responses. Also, miRNAs are dysregulated in smoking-associated diseases. We investigated the miRNA profile of 523 miRNAs by stem-loop RT-qPCR in lung tissue and cell-free bronchoalveolar lavage (BAL) supernatant of mice exposed to air or cigarette smoke (CS) for 4 or 24 weeks. After 24 weeks of CS exposure, 31 miRNAs were differentially expressed in lung tissue and 78 in BAL supernatant. Next, we correlated the miRNA profiling data to inflammation in BAL and lung, obtained by flow cytometry or ELISA. In addition, we surveyed for overlap with newly assessed miRNA profiles in bronchial biopsies and with previously assessed miRNA profiles in lung tissue and induced sputum supernatant of smokers with COPD. Several miRNAs showed concordant differential expression between both species including miR-31, miR-155, miR-218 and let-7c. Thus, investigating miRNA profiling data in different compartments and both species provided accumulating insights in miRNAs that may be relevant in CS-induced inflammation and the pathogenesis of COPD.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/s41598-017-13265-8	en_US
dc.subject.mesh	Lung	en_US
dc.subject.mesh	Bronchoalveolar Lavage Fluid	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred C57BL	en_US
dc.subject.mesh	Pulmonary Disease, Chronic Obstructive	en_US
dc.subject.mesh	Inflammation	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Statistics, Nonparametric	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Smoking	en_US
dc.subject.mesh	Air	en_US
dc.subject.mesh	Down-Regulation	en_US
dc.subject.mesh	Up-Regulation	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Translational Medical Research	en_US
dc.title	MicroRNA profiling in lung tissue and bronchoalveolar lavage of cigarette smoke-exposed mice and in COPD patients: A translational approach	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	7	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

© 2017 The Author(s). Chronic obstructive pulmonary disease (COPD) is characterized by a progressive airflow limitation and is associated with a chronic inflammatory response in both airways and lungs. microRNAs (miRNAs) are often highly conserved between species and have an intricate role within homeostatic conditions and immune responses. Also, miRNAs are dysregulated in smoking-associated diseases. We investigated the miRNA profile of 523 miRNAs by stem-loop RT-qPCR in lung tissue and cell-free bronchoalveolar lavage (BAL) supernatant of mice exposed to air or cigarette smoke (CS) for 4 or 24 weeks. After 24 weeks of CS exposure, 31 miRNAs were differentially expressed in lung tissue and 78 in BAL supernatant. Next, we correlated the miRNA profiling data to inflammation in BAL and lung, obtained by flow cytometry or ELISA. In addition, we surveyed for overlap with newly assessed miRNA profiles in bronchial biopsies and with previously assessed miRNA profiles in lung tissue and induced sputum supernatant of smokers with COPD. Several miRNAs showed concordant differential expression between both species including miR-31, miR-155, miR-218 and let-7c. Thus, investigating miRNA profiling data in different compartments and both species provided accumulating insights in miRNAs that may be relevant in CS-induced inflammation and the pathogenesis of COPD.

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http://hdl.handle.net/10453/129314