Quantification method for elemental bio-imaging by LA-ICP-MS using metal spiked PMMA films

Royal Society of Chemistry
Publication Type:
Journal Article
Journal of Analytical Atomic Spectrometry, 2010, 25 (5), pp. 722 - 725
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A method for quantitative analysis of biological soft tissues by laser ablation-inductively coupled plasma-mass spectrometry has been developed. Polymer film standards were produced by spin coating spiked solutions of poly methyl methacrylate onto quartz substrates. Calibration curves throughout the range of 0--400"llg g-l yielded correlation coefficients better than 0.999 for 66Zn and 63CU. Spiked, homogenised soft tissue standards were quantified by LA-ICP-MS against the thin film standards. The results agreed with values calculated from solution nebulisation rCP-MS. A procedure for internal standardisation by employing ruthenium or yttrium in the underlying thin film was also assessed.
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