Age-related gene and miRNA expression changes in airways of healthy individuals

Ong, J; Woldhuis, RR; Boudewijn, IM; van den Berg, A; Kluiver, J; Kok, K; Terpstra, MM; Guryev, V; de Vries, M; Vermeulen, CJ; Timens, W; van den Berge, M; Brandsma, CA

Age-related gene and miRNA expression changes in airways of healthy individuals

Ong, J Woldhuis, RR

Boudewijn, IM van den Berg, A Kluiver, J Kok, K Terpstra, MM Guryev, V de Vries, M Vermeulen, CJ Timens, W van den Berge, M Brandsma, CA

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2019, 9 (1)
Issue Date:: 2019-12-01

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Field	Value	Language
dc.contributor.author	Ong, J	en_US
dc.contributor.author	Woldhuis, RR https://orcid.org/0000-0001-7516-1034	en_US
dc.contributor.author	Boudewijn, IM	en_US
dc.contributor.author	van den Berg, A	en_US
dc.contributor.author	Kluiver, J	en_US
dc.contributor.author	Kok, K	en_US
dc.contributor.author	Terpstra, MM	en_US
dc.contributor.author	Guryev, V	en_US
dc.contributor.author	de Vries, M	en_US
dc.contributor.author	Vermeulen, CJ	en_US
dc.contributor.author	Timens, W	en_US
dc.contributor.author	van den Berge, M	en_US
dc.contributor.author	Brandsma, CA	en_US
dc.date.available	2019-02-01	en_US
dc.date.issued	2019-12-01	en_US
dc.identifier.citation	Scientific Reports, 2019, 9 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136036
dc.description.abstract	© 2019, The Author(s). Knowledge on age-related miRNA changes in healthy individuals and their interaction with mRNAs is lacking. We studied age-related mRNA and miRNA expression changes and their interactions in normal airways. RNA and small RNA sequencing was performed on bronchial biopsies of 86 healthy individuals (age: 18–73) to determine age-related expression changes. Per age-related miRNA we determined the enrichment of age-related predicted targets and their correlation. We identified 285 age-related genes and 27 age-related miRNAs. Pathway enrichment showed that genes higher expressed with age were involved in synapse-related processes. Genes lower expressed with age were involved in cell cycle regulation, the immune system and DNA damage/repair. MiR-146a-5p, miR-146b-5p and miR-142-5p were lower expressed with increasing age and we found a significant enrichment for predicted targets of these miRNAs among genes that were higher expressed with age. The expression levels of the enriched predicted targets RIMS2 and IGSF1 were negatively correlated with both miR-146a-5p and miR-146b-5p. RIMS2 was present in the enriched process, i.e. positive regulation of synaptic transmission. In conclusion, genes decreased with ageing are involved in several of the ageing hallmarks. Genes higher expressed with ageing were involved in synapse-related processes, of which RIMS2 is potentially regulated by two age-related miRNAs.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/s41598-019-39873-0	en_US
dc.title	Age-related gene and miRNA expression changes in airways of healthy individuals	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	9	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

© 2019, The Author(s). Knowledge on age-related miRNA changes in healthy individuals and their interaction with mRNAs is lacking. We studied age-related mRNA and miRNA expression changes and their interactions in normal airways. RNA and small RNA sequencing was performed on bronchial biopsies of 86 healthy individuals (age: 18–73) to determine age-related expression changes. Per age-related miRNA we determined the enrichment of age-related predicted targets and their correlation. We identified 285 age-related genes and 27 age-related miRNAs. Pathway enrichment showed that genes higher expressed with age were involved in synapse-related processes. Genes lower expressed with age were involved in cell cycle regulation, the immune system and DNA damage/repair. MiR-146a-5p, miR-146b-5p and miR-142-5p were lower expressed with increasing age and we found a significant enrichment for predicted targets of these miRNAs among genes that were higher expressed with age. The expression levels of the enriched predicted targets RIMS2 and IGSF1 were negatively correlated with both miR-146a-5p and miR-146b-5p. RIMS2 was present in the enriched process, i.e. positive regulation of synaptic transmission. In conclusion, genes decreased with ageing are involved in several of the ageing hallmarks. Genes higher expressed with ageing were involved in synapse-related processes, of which RIMS2 is potentially regulated by two age-related miRNAs.

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http://hdl.handle.net/10453/136036