Distinct morphological fates of uropathogenic E. coli intracellular bacterial communities: dependency on urine composition and pH.

Iosifidis, G; Duggin, IG

Distinct morphological fates of uropathogenic E. coli intracellular bacterial communities: dependency on urine composition and pH.

Iosifidis, G Duggin, IG

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Publisher:: American Society for Microbiology
Publication Type:: Journal Article
Citation:: Infection and immunity, 2020
Issue Date:: 2020-06-15

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Field	Value	Language
dc.contributor.author	Iosifidis, G
dc.contributor.author	Duggin, IG
dc.date.accessioned	2020-06-27T11:47:01Z
dc.date.available	2020-06-27T11:47:01Z
dc.date.issued	2020-06-15
dc.identifier.citation	Infection and immunity, 2020
dc.identifier.issn	0019-9567
dc.identifier.issn	1098-5522
dc.identifier.uri	http://hdl.handle.net/10453/141678
dc.description.abstract	Uropathogenic E. coli (UPEC) is the leading cause of urinary tract infections (UTI). These bacteria undertake a multi-stage infection cycle involving invasion of and proliferation within urinary tract epithelial cells, leading to the rupture of the host cell and dispersal of the bacteria, some of which have a highly filamentous morphology. Here we established a microfluidics-based model of UPEC infection of immortalized human bladder epithelial cells that recapitulates the main stages of bacterial morphological changes during the acute infection cycle in vivo and allows the development and fate of individual cells to be monitored in real-time by fluorescence microscopy. The UPEC-infected bladder cells remained alive and mobile in non-confluent monolayers during the development of intracellular bacterial communities (IBCs). Switching from a flow of growth medium to human urine resulted in immobilization of both uninfected and infected bladder cells. IBCs continued to develop and then released many highly filamentous bacteria via an extrusion-like process, whereas others showed strong UPEC proliferation yet no detected filamentation. The filamentation response was dependent on the weak acidity of human urine and required component(s) in a low molecular-mass (<3000 Da) fraction from a mildly dehydrated donor. The developmental fate for bacteria therefore appears to be controlled by multiple factors that act at the level of the whole IBC, suggesting that variable local environments or stochastic differentiation pathways influence IBC developmental fates during infection.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	American Society for Microbiology
dc.relation	http://purl.org/au-research/grants/arc/FT160100010
dc.relation.ispartof	Infection and immunity
dc.relation.hasversion	Accepted manuscript version	en
dc.relation.isbasedon	10.1128/iai.00884-19
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	06 Biological Sciences, 07 Agricultural and Veterinary Sciences, 11 Medical and Health Sciences
dc.subject.classification	Microbiology
dc.title	Distinct morphological fates of uropathogenic E. coli intracellular bacterial communities: dependency on urine composition and pH.
dc.type	Journal Article
utslib.location.activity	United States
utslib.for	06 Biological Sciences
utslib.for	07 Agricultural and Veterinary Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	recently_added	*
dc.date.updated	2020-06-27T11:46:56Z
pubs.publication-status	Published

Abstract:

Uropathogenic E. coli (UPEC) is the leading cause of urinary tract infections (UTI). These bacteria undertake a multi-stage infection cycle involving invasion of and proliferation within urinary tract epithelial cells, leading to the rupture of the host cell and dispersal of the bacteria, some of which have a highly filamentous morphology. Here we established a microfluidics-based model of UPEC infection of immortalized human bladder epithelial cells that recapitulates the main stages of bacterial morphological changes during the acute infection cycle in vivo and allows the development and fate of individual cells to be monitored in real-time by fluorescence microscopy. The UPEC-infected bladder cells remained alive and mobile in non-confluent monolayers during the development of intracellular bacterial communities (IBCs). Switching from a flow of growth medium to human urine resulted in immobilization of both uninfected and infected bladder cells. IBCs continued to develop and then released many highly filamentous bacteria via an extrusion-like process, whereas others showed strong UPEC proliferation yet no detected filamentation. The filamentation response was dependent on the weak acidity of human urine and required component(s) in a low molecular-mass (<3000 Da) fraction from a mildly dehydrated donor. The developmental fate for bacteria therefore appears to be controlled by multiple factors that act at the level of the whole IBC, suggesting that variable local environments or stochastic differentiation pathways influence IBC developmental fates during infection.

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http://hdl.handle.net/10453/141678