Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage.

Hamidian, M; Blasco, L; Tillman, LN; To, J; Tomas, M; Myers, GSA

Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage.

Hamidian, M

Blasco, L Tillman, LN To, J Tomas, M Myers, GSA

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Microorganisms, 2020, 8, (12), pp. 1851-1851
Issue Date:: 2020-11-24

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Field	Value	Language
dc.contributor.author	Hamidian, M https://orcid.org/0000-0002-3614-7261
dc.contributor.author	Blasco, L
dc.contributor.author	Tillman, LN
dc.contributor.author	To, J
dc.contributor.author	Tomas, M
dc.contributor.author	Myers, GSA
dc.date.accessioned	2020-12-03T00:36:56Z
dc.date.available	2020-11-21
dc.date.available	2020-12-03T00:36:56Z
dc.date.issued	2020-11-24
dc.identifier.citation	Microorganisms, 2020, 8, (12), pp. 1851-1851
dc.identifier.issn	2076-2607
dc.identifier.issn	2076-2607
dc.identifier.uri	http://hdl.handle.net/10453/144480
dc.description.abstract	<i>Acinetobacter baumannii</i> isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of <i>A. baumannii</i>, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn<i>6551</i>. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI AG
dc.relation	http://purl.org/au-research/grants/arc/DE200100111
dc.relation.ispartof	Microorganisms
dc.relation.isbasedon	10.3390/microorganisms8121851
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage.
dc.type	Journal Article
utslib.citation.volume	8
utslib.location.activity	Switzerland
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-12-03T00:36:33Z
pubs.issue	12
pubs.publication-status	Published
pubs.volume	8
utslib.citation.issue	12

Abstract:

Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn6551. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type.

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http://hdl.handle.net/10453/144480

Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage.