Uptake of dimethylsulphoniopropionate (DMSP) by the diatom Thalassiosira weissflogii: a model to investigate the cellular function of DMSP

Petrou, K; D. A. Nielsen,

Uptake of dimethylsulphoniopropionate (DMSP) by the diatom Thalassiosira weissflogii: a model to investigate the cellular function of DMSP

Petrou, K

D. A. Nielsen,

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Publisher:: SPRINGER
Publication Type:: Journal Article
Citation:: Biogeochemistry., 2018, 141, (2), pp. 265-271
Issue Date:: 2018-11

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Field	Value	Language
dc.contributor.author	Petrou, K https://orcid.org/0000-0002-2703-0694
dc.contributor.author	D. A. Nielsen,
dc.date.accessioned	2021-03-31T07:57:49Z
dc.date.available	2021-03-31T07:57:49Z
dc.date.issued	2018-11
dc.identifier.citation	Biogeochemistry., 2018, 141, (2), pp. 265-271
dc.identifier.issn	0168-2563
dc.identifier.issn	1573-515X
dc.identifier.uri	http://hdl.handle.net/10453/147726
dc.description.abstract	One of the most abundant organic sulphur molecules in the ocean, dimethylsulphoniopropionate (DMSP) has been implicated in numerous biochemical functions and ecological interactions, from osmotic and oxidative stress regulation within the cell, to the chemical attraction of bacteria, mammals and birds in the environment. Notwithstanding these varied and important discoveries, the primary role of DMSP in the cell remains elusive. In this study, we take a new approach to investigating the role of DMSP in cell physiology. Rather than utilising a known DMSP-producer, we instead exploit the propensity for the non-DMSP producing diatom Thalassiosira weissflogii to take up DMSP from its environment. We characterise the uptake and retention of the molecule under growth conditions and salinity stress with the aim to elucidate its utility as a model system for investigating the cellular function of DMSP. Thalassiosira weissflogii showed concentration-dependent uptake of DMSP and complete retention within the cell for at least 6 h. Saturation of intracellular DMSP occurred at > 87 mM, equivalent to some of the most prolific DMSP-producing species. Salinity shifts resulted in a reduction in DMSP uptake rate, but only at extremely low (17) or very high (45) salinities. These data demonstrate the potential for using T. weissflogii in physiological studies, providing a true (DMSP-free) control, as well as a DMSP-enriched version of the same strain. In this way, orthogonal experiments may be conducted with the aim to uncover the physiological purpose of DMSP in phytoplankton and potentially add key pieces to the enigmatic DMSP puzzle.
dc.format	Undetermined
dc.language	eng
dc.publisher	SPRINGER
dc.relation.ispartof	Biogeochemistry.
dc.relation.isbasedon	10.1007/s10533-018-0507-1
dc.rights	This is a post-peer-review, pre-copyedit version of an article published in [Biogeochemistry., 2018, 141, (2), pp. 265-271]. The final authenticated version is available online at: https://link.springer.com/article/10.1007/s10533-018-0507-1]”
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0399 Other Chemical Sciences, 0402 Geochemistry, 0502 Environmental Science and Management
dc.subject.classification	Agronomy & Agriculture
dc.title	Uptake of dimethylsulphoniopropionate (DMSP) by the diatom Thalassiosira weissflogii: a model to investigate the cellular function of DMSP
dc.type	Journal Article
utslib.citation.volume	141
utslib.for	0399 Other Chemical Sciences
utslib.for	0399 Other Chemical Sciences
utslib.for	0402 Geochemistry
utslib.for	0502 Environmental Science and Management
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2021-03-31T07:57:47Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	141
utslib.citation.issue	2

Abstract:

One of the most abundant organic sulphur molecules in the ocean, dimethylsulphoniopropionate (DMSP) has been implicated in numerous biochemical functions and ecological interactions, from osmotic and oxidative stress regulation within the cell, to the chemical attraction of bacteria, mammals and birds in the environment. Notwithstanding these varied and important discoveries, the primary role of DMSP in the cell remains elusive. In this study, we take a new approach to investigating the role of DMSP in cell physiology. Rather than utilising a known DMSP-producer, we instead exploit the propensity for the non-DMSP producing diatom Thalassiosira weissflogii to take up DMSP from its environment. We characterise the uptake and retention of the molecule under growth conditions and salinity stress with the aim to elucidate its utility as a model system for investigating the cellular function of DMSP. Thalassiosira weissflogii showed concentration-dependent uptake of DMSP and complete retention within the cell for at least 6 h. Saturation of intracellular DMSP occurred at > 87 mM, equivalent to some of the most prolific DMSP-producing species. Salinity shifts resulted in a reduction in DMSP uptake rate, but only at extremely low (17) or very high (45) salinities. These data demonstrate the potential for using T. weissflogii in physiological studies, providing a true (DMSP-free) control, as well as a DMSP-enriched version of the same strain. In this way, orthogonal experiments may be conducted with the aim to uncover the physiological purpose of DMSP in phytoplankton and potentially add key pieces to the enigmatic DMSP puzzle.

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http://hdl.handle.net/10453/147726