Identification of asthma associated microRNAs in bronchial biopsies.

Roffel, MP; Boudewijn, IM; van Nijnatten, JLL; Faiz, A; Vermeulen, CJ; van Oosterhout, AJ; Affleck, K; Timens, W; Bracke, KR; Maes, T; Heijink, IH; Brandsma, C-A; van den Berge, M

Identification of asthma associated microRNAs in bronchial biopsies.

Roffel, MP Boudewijn, IM van Nijnatten, JLL Faiz, A

Vermeulen, CJ van Oosterhout, AJ Affleck, K Timens, W Bracke, KR Maes, T Heijink, IH Brandsma, C-A van den Berge, M

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Publisher:: EUROPEAN RESPIRATORY SOC JOURNALS LTD
Publication Type:: Journal Article
Citation:: Eur Respir J, 2022, 59, (3), pp. 2101294
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Roffel, MP
dc.contributor.author	Boudewijn, IM
dc.contributor.author	van Nijnatten, JLL
dc.contributor.author	Faiz, A https://orcid.org/0000-0003-1740-3538
dc.contributor.author	Vermeulen, CJ
dc.contributor.author	van Oosterhout, AJ
dc.contributor.author	Affleck, K
dc.contributor.author	Timens, W
dc.contributor.author	Bracke, KR
dc.contributor.author	Maes, T
dc.contributor.author	Heijink, IH
dc.contributor.author	Brandsma, C-A
dc.contributor.author	van den Berge, M
dc.date.accessioned	2023-02-05T23:49:00Z
dc.date.available	2021-07-30
dc.date.available	2023-02-05T23:49:00Z
dc.date.issued	2022-01-01
dc.identifier.citation	Eur Respir J, 2022, 59, (3), pp. 2101294
dc.identifier.issn	0903-1936
dc.identifier.issn	1399-3003
dc.identifier.uri	http://hdl.handle.net/10453/165909
dc.description.abstract	Changes in microRNA (miRNA) expression can contribute to the pathogenesis of many diseases, including asthma. We aimed to identify miRNAs that are differentially expressed between asthma patients and healthy controls and explored their association with clinical and inflammatory parameters of asthma.Differentially expressed miRNAs were determined by small RNA sequencing on bronchial biopsies of 79 asthma patients and 82 healthy controls using linear regression models. Differentially expressed miRNAs were associated with clinical and inflammatory asthma features. Potential miRNA-mRNA interactions were analysed using mRNA data available from the same bronchial biopsies and enrichment of pathways was identified with Enrichr and g:Profiler.In total 78 differentially expressed miRNAs were identified in bronchial biopsies of asthma patients compared to controls, of which 60 remained differentially expressed after controlling for smoke and inhaled corticosteroid treatment. We identified several asthma associated miRNAs, including miR-125b-5p and miR-223-3p, based on a significant association with multiple clinical and inflammatory asthma features and their negative correlation with genes associated with the presence of asthma. The most enriched biological pathway(s) affected by miR-125b-5p and miR-223-3p were inflammatory response and cilium assembly and organisation. Of interest, we identified that lower expression of miR-26a-5p was linked to more severe eosinophilic inflammation as measured in blood, sputum as well as bronchial biopsies. Collectively, we identified miR-125b-5p, miR-223-3p and miR-26a-5p, as potential regulators that could contribute to the pathogenesis of asthma.
dc.format	Electronic-Print
dc.language	eng
dc.publisher	EUROPEAN RESPIRATORY SOC JOURNALS LTD
dc.relation.ispartof	Eur Respir J
dc.relation.isbasedon	10.1183/13993003.01294-2021
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	11 Medical and Health Sciences, 1116 Medical Physiology
dc.subject.classification	Respiratory System
dc.subject.mesh	Asthma
dc.subject.mesh	Biopsy
dc.subject.mesh	Eosinophilia
dc.subject.mesh	Gene Expression Profiling
dc.subject.mesh	Humans
dc.subject.mesh	MicroRNAs
dc.subject.mesh	Sputum
dc.subject.mesh	Sputum
dc.subject.mesh	Humans
dc.subject.mesh	Asthma
dc.subject.mesh	Eosinophilia
dc.subject.mesh	MicroRNAs
dc.subject.mesh	Biopsy
dc.subject.mesh	Gene Expression Profiling
dc.title	Identification of asthma associated microRNAs in bronchial biopsies.
dc.type	Journal Article
utslib.citation.volume	59
utslib.location.activity	England
utslib.for	11 Medical and Health Sciences
utslib.for	1116 Medical Physiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
pubs.organisational-group	/University of Technology Sydney/Strength - CFI - Centre for Inflammation
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2023-02-05T23:48:59Z
pubs.issue	3
pubs.publication-status	Published online
pubs.volume	59
utslib.citation.issue	3

Abstract:

Changes in microRNA (miRNA) expression can contribute to the pathogenesis of many diseases, including asthma. We aimed to identify miRNAs that are differentially expressed between asthma patients and healthy controls and explored their association with clinical and inflammatory parameters of asthma.Differentially expressed miRNAs were determined by small RNA sequencing on bronchial biopsies of 79 asthma patients and 82 healthy controls using linear regression models. Differentially expressed miRNAs were associated with clinical and inflammatory asthma features. Potential miRNA-mRNA interactions were analysed using mRNA data available from the same bronchial biopsies and enrichment of pathways was identified with Enrichr and g:Profiler.In total 78 differentially expressed miRNAs were identified in bronchial biopsies of asthma patients compared to controls, of which 60 remained differentially expressed after controlling for smoke and inhaled corticosteroid treatment. We identified several asthma associated miRNAs, including miR-125b-5p and miR-223-3p, based on a significant association with multiple clinical and inflammatory asthma features and their negative correlation with genes associated with the presence of asthma. The most enriched biological pathway(s) affected by miR-125b-5p and miR-223-3p were inflammatory response and cilium assembly and organisation. Of interest, we identified that lower expression of miR-26a-5p was linked to more severe eosinophilic inflammation as measured in blood, sputum as well as bronchial biopsies. Collectively, we identified miR-125b-5p, miR-223-3p and miR-26a-5p, as potential regulators that could contribute to the pathogenesis of asthma.

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http://hdl.handle.net/10453/165909