An engineered membrane to measure electroporation: Effect of tethers and bioelectronic interface

Hoiles, W; Krishnamurthy, V; Cranfield, CG; Cornell, B

An engineered membrane to measure electroporation: Effect of tethers and bioelectronic interface

Hoiles, W Krishnamurthy, V Cranfield, CG

Cornell, B

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Publication Type:: Journal Article
Citation:: Biophysical Journal, 2014, 107 (6), pp. 1339 - 1351
Issue Date:: 2014-09-16

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Field	Value	Language
dc.contributor.author	Hoiles, W	en_US
dc.contributor.author	Krishnamurthy, V	en_US
dc.contributor.author	Cranfield, CG https://orcid.org/0000-0003-3608-5440	en_US
dc.contributor.author	Cornell, B https://orcid.org/0000-0003-4166-3241	en_US
dc.date.available	2014-07-30	en_US
dc.date.issued	2014-09-16	en_US
dc.identifier.citation	Biophysical Journal, 2014, 107 (6), pp. 1339 - 1351	en_US
dc.identifier.issn	0006-3495	en_US
dc.identifier.uri	http://hdl.handle.net/10453/30152
dc.description.abstract	© 2014 by the Biophysical Society. This article reports on the construction and predictive models for a platform comprised of an engineered tethered membrane. The platform provides a controllable and physiologically relevant environment for the study of the electroporation process. The mixed self-assembled membrane is formed via a rapid solvent exchange technique. The membrane is tethered to the gold electrode and includes an ionic reservoir separating the membrane and gold surface. Above the membrane, there is an electrolyte solution, and a gold counterelectrode. A voltage is applied between the gold electrodes and the current measured. The current is dependent on the energy required to form aqueous pores and the conductance of each pore. A two-level predictive model, consisting of a macroscopic and a continuum model, is developed to relate the pore dynamics to the measured current. The macroscopic model consists of an equivalent circuit model of the tethered membrane, and asymptotic approximations to the Smoluchowski-Einstein equation of electroporation that is dependent on the pore conductance and the energy required to form aqueous pores. The continuum model is a generalized Poisson-Nernst-Planck (GPNP) system where an activity coefficient to account for steric effects of ions is added to the standard PNP system. The GPNP is used to evaluate the conductance of aqueous pores, and the electrical energy required to form the pores. As an outcome of the setup of the device and the two-level model, biologically important variables can be estimated from experimental measurements. To validate the accuracy of the two-level model, the predicted current is compared with experimentally measured current for different tethering densities.	en_US
dc.relation.ispartof	Biophysical Journal	en_US
dc.relation.isbasedon	10.1016/j.bpj.2014.07.056	en_US
dc.subject.classification	Biophysics	en_US
dc.subject.mesh	Water	en_US
dc.subject.mesh	Membranes, Artificial	en_US
dc.subject.mesh	Electroporation	en_US
dc.subject.mesh	Poisson Distribution	en_US
dc.subject.mesh	Electrodes	en_US
dc.subject.mesh	Porosity	en_US
dc.subject.mesh	Models, Biological	en_US
dc.subject.mesh	Engineering	en_US
dc.title	An engineered membrane to measure electroporation: Effect of tethers and bioelectronic interface	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	107	en_US
utslib.for	060110 Receptors and Membrane Biology	en_US
utslib.for	060601 Animal Physiology - Biophysics	en_US
utslib.for	02 Physical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	107	en_US

Abstract:

© 2014 by the Biophysical Society. This article reports on the construction and predictive models for a platform comprised of an engineered tethered membrane. The platform provides a controllable and physiologically relevant environment for the study of the electroporation process. The mixed self-assembled membrane is formed via a rapid solvent exchange technique. The membrane is tethered to the gold electrode and includes an ionic reservoir separating the membrane and gold surface. Above the membrane, there is an electrolyte solution, and a gold counterelectrode. A voltage is applied between the gold electrodes and the current measured. The current is dependent on the energy required to form aqueous pores and the conductance of each pore. A two-level predictive model, consisting of a macroscopic and a continuum model, is developed to relate the pore dynamics to the measured current. The macroscopic model consists of an equivalent circuit model of the tethered membrane, and asymptotic approximations to the Smoluchowski-Einstein equation of electroporation that is dependent on the pore conductance and the energy required to form aqueous pores. The continuum model is a generalized Poisson-Nernst-Planck (GPNP) system where an activity coefficient to account for steric effects of ions is added to the standard PNP system. The GPNP is used to evaluate the conductance of aqueous pores, and the electrical energy required to form the pores. As an outcome of the setup of the device and the two-level model, biologically important variables can be estimated from experimental measurements. To validate the accuracy of the two-level model, the predicted current is compared with experimentally measured current for different tethering densities.

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http://hdl.handle.net/10453/30152