The quest for improved reproducibility in MALDI mass spectrometry

O'Rourke, MB; Djordjevic, SP; Padula, MP

The quest for improved reproducibility in MALDI mass spectrometry

O'Rourke, MB

Djordjevic, SP

Padula, MP

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Publication Type:: Journal Article
Citation:: Mass Spectrometry Reviews, 2018, 37 (2), pp. 217 - 228
Issue Date:: 2018-03-01

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Field	Value	Language
dc.contributor.author	O'Rourke, MB https://orcid.org/0000-0002-0552-2962	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Padula, MP https://orcid.org/0000-0002-8283-0643	en_US
dc.date.available	2020-05-25T19:13:50Z
dc.date.issued	2018-03-01	en_US
dc.identifier.citation	Mass Spectrometry Reviews, 2018, 37 (2), pp. 217 - 228	en_US
dc.identifier.issn	0277-7037	en_US
dc.identifier.uri	http://hdl.handle.net/10453/44191
dc.description.abstract	© 2016 Wiley Periodicals, Inc. Reproducibility has been one of the biggest hurdles faced when attempting to develop quantitative protocols for MALDI mass spectrometry. The heterogeneous nature of sample recrystallization has made automated sample acquisition somewhat “hit and miss” with manual intervention needed to ensure that all sample spots have been analyzed. In this review, we explore the last 30 years of literature and anecdotal evidence that has attempted to address and improve reproducibility in MALDI MS. Though many methods have been attempted, we have discovered a significant publication history surrounding the use of nitrocellulose as a substrate to improve homogeneity of crystal formation and therefore reproducibility. We therefore propose that this is the most promising avenue of research for developing a comprehensive and universal preparation protocol for quantitative MALDI MS analysis. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:217–228, 2018.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LP0348851
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT9000057
dc.relation	http://purl.org/au-research/grants/arc/LE110100078
dc.relation	http://purl.org/au-research/grants/arc/LE130100096
dc.relation	http://purl.org/au-research/grants/arc/LE130100007
dc.relation	http://purl.org/au-research/grants/arc/LP150100912
dc.relation	http://purl.org/au-research/grants/arc/LE150100031
dc.relation	http://purl.org/au-research/grants/arc/LE160100204
dc.relation.ispartof	Mass Spectrometry Reviews	en_US
dc.relation.isbasedon	10.1002/mas.21515	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.title	The quest for improved reproducibility in MALDI mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	37	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	37	en_US

Abstract:

© 2016 Wiley Periodicals, Inc. Reproducibility has been one of the biggest hurdles faced when attempting to develop quantitative protocols for MALDI mass spectrometry. The heterogeneous nature of sample recrystallization has made automated sample acquisition somewhat “hit and miss” with manual intervention needed to ensure that all sample spots have been analyzed. In this review, we explore the last 30 years of literature and anecdotal evidence that has attempted to address and improve reproducibility in MALDI MS. Though many methods have been attempted, we have discovered a significant publication history surrounding the use of nitrocellulose as a substrate to improve homogeneity of crystal formation and therefore reproducibility. We therefore propose that this is the most promising avenue of research for developing a comprehensive and universal preparation protocol for quantitative MALDI MS analysis. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:217–228, 2018.

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http://hdl.handle.net/10453/44191