Isolation of Toxoplasma gondii from the brain of a dog in Australia and its biological and molecular characterization

Publisher:
Elsevier
Publication Type:
Journal Article
Citation:
Veterinary Parasitology, 2009, 164 (2-4), pp. 335 - 339
Issue Date:
2009-01
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Toxoplasma gondii was isolated from the brain of a young dog for the ?rst time in Australia. The identity of the parasite was con?rmed by PCR, Western blotting, electron microscopy and cat bioassay. Genotyping of the isolate (TgDgAu1) was determined by PCR-RFLP markers that showed it to be a Type II strain. Western blotting demonstrated the presence of IgM antibodies to T. gondii suggesting the bitch was probably infected during pregnancy and the T. gondii was transmitted to the pups congenitally. We believe this represents the ?rst description of a natural case of congenital transmission of T. gondii in the dog
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